덱사메타손이 신생 돼지 췌장 세포 이식 후 증식과 베타 세포로의 분화에 미치는 영향
The Effects of Dexamethasone on the Expansion and Transdifferentiation of Transplanted Porcine Neonatal Pancreas Cell Clustersinto beta-cells in Normal Nude Mice
Background: Several studies have suggested that glucocorticoid has an influence on the development and function of the β-cells. Thus, we undertook this study to determine whether exposure to dexamethasone(Dx) has an influence on the expansion or transdifferentiation of transplanted porcine NPCCs. Methods: After transplantation(Tx) of 4,000 islet equivalents(IEQs) of porcine NPCCs into normal nude mice, Dx(1mg/kg) or the control vehicle were injected daily for 10 weeks. To clarify the effects of timing and duration of the Dx, one group was treated by Dx at the first 2 weeks(n=10) and the other group was treated later 8 weeks(n=10) during the 10 weeks treatment period. Thr total graft and β-cell masses were determined by morphometric analysis. We preformed semi-quantitative RT-PCR for evaluating the pancreas transcription factors. Results: The relative volume and absolute mass of the β-cells and the total graft were significantly decreased by 10 weeks Dx treatment. Moreover, Dx treatment at thr first 2 weeks(n=10) also significantly decreased the total graft mass and absolute mass of the β-cells. The relative volume of the β-cells was negatively correlated and the area of the duct cysts was positively correlated with the duration of the Dx treatment. Pancreas transcription factors including PDX1, Ngn 3, ISL1 and NKx6.1 were decreased in the graft by 2 days treatment of Dx.Conclusion: These results suggest that Dx treatment suppresses the expansion and transdifferentiation of transplanted pancreas precursor cells into β-cell(J Kor Diabetes Assoc 28:356~366, 2004).
- 원문이 없습니다.
- 원문이 없습니다.
유료 다운로드의 경우 해당 사이트의 정책에 따라 신규 회원가입, 로그인, 유료 구매 등이 필요할 수 있습니다. 해당 사이트에서 발생하는 귀하의 모든 정보활동은 NDSL의 서비스 정책과 무관합니다.
원문복사신청을 하시면, 일부 해외 인쇄학술지의 경우 외국학술지지원센터(FRIC)에서
무료 원문복사 서비스를 제공합니다.