송라차의 성분인 usnic acid에 의한 대장암 세포의 증식 억제 및 세포 사멸 효과
Inhibition of Cell Proliferation and Apoptosis induced by Usnic acid, a Naturally Occurring Compound of 'Song Luo' Tea, in Human Colon Cancer SW480 and HCT116 Cells
This study was carried out to investigate the effect of usnic acid on human colon cancer cells. Treatment with various concentrations of usnic acid for 24 and 48 h significantly inhibited the proliferation of SW480 and HCT116 cells. Usnic acid showed a stronger antiproliferative effect on SW480 than on HCT116. To ascertain the underlying mechanism of this antiproliferation by usnic acid, the observation of the stained cells by an inverted microscope was performed with an APOPercentage Apoptosis Assay kit. It was found that the number of purple-red stained cells in the SW480 and HCT116 cells increased in an usnic acid dose-dependent manner and that the discrepancy in the number of stained cells between the two cell types showed a similar trend to the results of the MTT assay. The double staining method was used for the characterization of the apoptotic stages according to the concentration of usnic acid. The results obtained with the FACS showed that the proportion of cells at the late apoptotic stage increased with increasing concentration of usnic acid in the SW480 and HCT116 cells. To elucidate mechanism of apoptosis, western blotting of the apoptosis related proteins was performed. It was found that the level of Bax was markedly increased and that of Bcl-2 was significantly decreased. Also it was revealed that usnic acid induced a cleaved form of caspase-3 and -9 at a high dosage level.
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