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벼(Oryza sativa)의 카드뮴에 대한 내성 기작 원문보기
Putative Mechanism of Cadmium Tolerance in Rice Cultivar(Oryza sativa)

  • 저자

    이소은

  • 학위수여기관

    이화여자대학교 대학원

  • 학위구분

    국내석사

  • 학과

    생물과학과

  • 지도교수

  • 발행년도

    2001

  • 총페이지

    vi, 64p.

  • 키워드

    벼 Oryza sativa 카드뮴;

  • 언어

    kor

  • 원문 URL

    http://www.riss.kr/link?id=T8951878&outLink=K  

  • 초록

    카드뮴(Cd)은 토양의 중금속 오염 중 먹이사슬에 의한 생물농축으로 인체 위해도가 높은 것으로 알려져 있으며, 본 연구는 다양한 벼품종들간의 서로 다른 카드뮴 내성기작을 규명하기 위해서 이루어졌다. 국내의 7개의 내염성 벼품종들을 5μM CdSO₄가 포함된 Hoagland's solution에 24일간 노출시켜 뿌리와 지상부의 생체량 및 길이 감소율로 내성을 측정하였고, 그 중 서로 다른 내성을 보이는 간척, 남원, 서진벼를 선발하였다. 간척 > 남원 > 서진벼 순서의 Cd에 대한 내성과 반대로 간척(577.5μgCd/gdw) 남원(19.40%) > 서진벼(13.69%) 순서로 높았다. Cd축적속도는 간척


    This research wasperformed to elucidate the mechanism of difference in tolerance to cadmium(Cd) among various lines of Oryza sativa. After examining Cd tolerances of 7 lines of rice reported to be salt-tolerant in Korea, three lines KanChuck, NamWon and SeoJin showing different range of Cd tolerance, were selected for further studies; the reduction rates of length of each line after culturing 24 days in Hoagland solution were 19.8% for KanChuck, 38% for NamWon, and 48.5% for SeoJin. To elucidate the mechanism of difference in Cd tolerance, the levels of accumulated cadmium in plants were measured; the root Cd concentrations (μg/g dw) of KanChuck, NamWon and SeoJin were 577.5, 862.5 and 1606.5 respectively. The meaningful difference, however, was not observed in glutathione levels which play a role in Cd detoxification. The root Cd accumulation rate (μgCd/g dw/h) of KanChuck, NamWon and SeoJin were 8.06, 9.37 and 12.66 respectively. And from Lineweaver-Bulk equation, a dual pattern of transport mechanisms were observed in NamWon and SeoJin root at the concentration range 0.5 to 4 μM Cd, but KanChuck root has one transport mechanism.Vmax of KanChuck root was lower than those of NamWon and SeoJin. Consequently the difference in cadmium tolerance among various lines of rice seems to be caused by different accumulation level of cadmium, suggesting that rice tolerate cadmium by decreasing cadmium accumulation, possibly by low uptake.


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