Pseudomonas sp. KL47에 의한 방향족 화합물의 분해 및 관련 유전자에 관한 연구
Genetic studies on the biodegradation of aromatic hydrocarbons by Pseudomonas sp. KL47
Pseudomonas sp KL47 방향족 화합물 분해 유전자 항생제;
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In a previous study, we have isolated Pseudomonas putida F1 mutants that could grow on biphenyl and propyl-, isopropyl-, butyl-, and sec-butylbenzene in addition to regular growth substrates, benzene, toluene and ethylbenzene. We also isolated Pseudomonas sp. KL47 from a contaminated soil and that can assimilate benzene, alkylbenzene (C1-C4), biphenyl, p-cumate and p-cymene. Experiments using GFP reporter vectors indicate that in strain KL47, cym and cmt operons are constitutively expressed and the tod operon is inducible by higher alkylbenzenes and biphenyl, the latter of which are not inducers for the tod operon in strain F1, as well as benzene, toluene and ethylbenzene. These results indicate that strain KL47 utilizes higher alkylbenzenes and biphenyl by hybrid catabolic pathways consisting of cmt and tod operons as determined previously. We have cloned the DNA fragments from KL47 genomic DNA library that support the growth of G7C.1 on toluene and p-cymene. The plasmids were named pEK6 and pEK27, respectively. We have sequenced pEK6(47,838 bp) and the result of analysis of pEK6 indicates that the nucleotide sequence of cloned genes is almost identical to those of strain F1. But TodS, a sensor protein of tod operon, has a mutation in 153 amino acid. Lys 153 of F1 is changed to Arg in TodS of KL47. We expect that the change may be important for recognition of broad effectors in KL47.