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2기압 환경에서 Human T Iymphocyte cell line(Jurkat cell)의 형태적 변화 및 단백채 분석 원문보기
Morphologic Changes and Proteomic analysis of Human T lymphocyte cell line (Jurkat cells) under 2 atmospheric pressure

  • 저자

    김웅

  • 학위수여기관

    高麗大學校 大學院

  • 학위구분

    국내박사

  • 학과

    의학과

  • 지도교수

  • 발행년도

    2004

  • 총페이지

    iii, 22p.

  • 키워드

    2기압 Human T Iymphocyte cell line Jurkat cell 단백채;

  • 언어

    kor

  • 원문 URL

    http://www.riss.kr/link?id=T10042567&outLink=K  

  • 초록

    There has been few report about cellular response under 2 atmospheric pressure (ATA) pressure in spite of many researches have been done for cellular response from mechanical stimuli including ultra high pressure (over 100 ATA pressure) and low-level of high pressure (under 1.5 ATA pressure). The study for cellular response under 2 ATA could give new result in cellular response from mechanical stimuli. To study cellular response from 2 ATA pressure the custom- designed pressure cell incubator was made and the CO_(2) level and temperature of pressure cell incubator were well controlled for cell incubation. The morphology of Jurkat cells were compared by SEM between the cells incubated in normal incubator and pressure incubator. The surface of Jurkat cells incubated in pressure chamber showed irregular, suggesting 2 ATA pressure gave enough stimuli to change cell surface. We also compared the protein changes by 2 D electrophoresis between the cells incubated in normal incubator and pressure incubator. The protein sptos were scanned and analysed by Imagemaster and changes of proteins were confirmed by Maldi-Tof analysis. The total of 10 proteins were changed in Jurkat cells by 2 ATA pressure. Among them, Adenosine deaminase, Tropomyosin 4, Tubulin-specific chaperone A, Protein kinase C, Thioredoxin peroxidase 2, Rho GDP-sissociation inhibitor 2, Hydroxyacyl-coenzyme dehydrogenase, Esterase D, 26S proteasome decreased and only mitochondrial HSP(heat shock proein) increased in pressurized Jurkat cells comparing to Jurkat cells under normal 1 ATA pressure.


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