난소 종양에서 osteopontin의 발현
Expression analysis of osteopontin in ovarian tumors
난소 종양 osteopontin;
- 원문 URL
Osteopontin, an integrin binding protein, has been involved in a variety of physiologic and pathologic conditions. Recently, osteopontin has been suggested to be a possible biomarker of ovarian cancer, but there are few reports on osteopontin expression of ovarian tumor tissues at present. To understand the expression pattern of osteopontin on various histologic types of ovarian tumors, we performed an immunohistochemical study on 90 paraffin embedded tissues including 80 ovarian epithelial tumors and 10 normal ovaries. We also performed a semiquantitative study of osteopontin mRNA on 12 fresh ovarian tumors, 2 normal ovaries and SKOV3 cell line. Among 55 carcinomas, over 50% of tumor cells expressed osteopontin in 14/14 clear cell, 11/12 endometrioid, 11/16 serous, and 5/15 mucinous types. The expression degrees were well correlated with the histologic types of carcinomas (P=0.002). Among 25 benign tumors, over 10% of tumor cells showed in 11/15 serous and 3/10 mucinous types (P=0.008). However, the degree of osteopontin expression was not related to the tumor grades or stages of ovarian cancer. Semiquantitative study for osteopontin mRNA showed 5∼6 times higher in SKOV3 cell line as well as in serous adenocarcinomas, compared with the normal ovaries. However, there was no increase of osteopontin mRNA in benign or malignant mucinous tumors. Our findings suggest that the expression of osteopontin is not only present in ovarian cancers, but also in benign ovarian tumors.