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감자(Solanum tuberosum)에 발생하는 Potato mop-top virus 분리동정 원문보기
Identification of Potato Mop-top virus from Solanum tuberosum cv. Gawon in Korea

  • 저자

    최정아

  • 학위수여기관

    경북대학교 대학원

  • 학위구분

    국내석사

  • 학과

    농생물학과 식물병리학전공

  • 지도교수

  • 발행년도

    2004

  • 총페이지

    21 p.

  • 키워드

    감자 바이러스병 감자;

  • 언어

    kor

  • 원문 URL

    http://www.riss.kr/link?id=T10045248&outLink=K  

  • 초록

    Potato mop-top virus(PMTV) was identified form Solanum tuberosum cv. Gawon showing bright chlorotic mottle symptom in Namwonm, Korea. Samples were collected in green-house in February, 2003. Electron microscopic examination of negatively stained preparation revealed that PMTV were rigid-rod shaped particles about 100-150, 250-300 nm ×18-20 nm in length. In ultrathin sections of leaf tissue form diseased potato plant, cluster of viruses particles was observed in the cytoplasm. TAS-ELISA determined that the virus was serologically related to PMTV. PMTV produced double ring necrotic local lesion in inoculated leaf of chenopodium amaranticolor when incubated at 15℃. PMTV could be detected with RT-PCR using PMTV detectable primer set designed to amplify about 540 bp of the partial CP gene of PMTV.


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