감자(Solanum tuberosum)에 발생하는 Potato mop-top virus 분리동정
Identification of Potato Mop-top virus from Solanum tuberosum cv. Gawon in Korea
감자 바이러스병 감자;
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Potato mop-top virus(PMTV) was identified form Solanum tuberosum cv. Gawon showing bright chlorotic mottle symptom in Namwonm, Korea. Samples were collected in green-house in February, 2003. Electron microscopic examination of negatively stained preparation revealed that PMTV were rigid-rod shaped particles about 100-150, 250-300 nm ×18-20 nm in length. In ultrathin sections of leaf tissue form diseased potato plant, cluster of viruses particles was observed in the cytoplasm. TAS-ELISA determined that the virus was serologically related to PMTV. PMTV produced double ring necrotic local lesion in inoculated leaf of chenopodium amaranticolor when incubated at 15℃. PMTV could be detected with RT-PCR using PMTV detectable primer set designed to amplify about 540 bp of the partial CP gene of PMTV.