Saccharomyces cerevisiae KNU5377의 열충격에 대한 막의 반응과 산화적 스트레스 반응 유도
Responding strategy of plasma membrane and Induction of oxidative stress response against heat shock in Saccharomyces cerevisiae KNU5377
열충격 Saccharomyces cerevisiae KNU5377;
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In yeast, heat stress is known to cause disorder of the membrane fatty acid composition and protein destruction in addition to an inhibition of glycolysis and an enhanced induction of trehalose. A high temperature increased unsaturation of membrane lipid and decreased its saturation in this yeast strain. This Saccharomyces cerevisiae KNU5377 showed a slight change in membrane lipid. And heat stress also increased heat shock protein synthesis and demolition or recovery of destructed protein. Among heat shock proteins, Hsp30 inhibited Pmap, H(+)-ATPase dependant transport protein, whose activity confers energy conservation. Heat shock stress impaired respiratory chain, and therefore, the resulting H_(2)O_(2)production was increased in the cells. Increased H_(2)O_(2) induced protein denaturation, lipid peroxidation and nucleus destruction. And it also induced oxidative stress and antioxidant enzyme activation(catalase and glutathione peroxidase). In 2D analysis, expression of high molecular protein was decreased and the one of low molecular protein was increased in heat shock stress.