난소 상피성 종양에서 Microsatellite instability 분석
Microsatellite alteration in benign, borderline and malignant epithelial tumors of the ovary
종양 난소암 난소;
- 원문 URL
Some genetic alterations are involved in ovarian carcinogenesis. Since some benign and borderline tumors may represent early steps in ovarian carcinogenesis, analysis of precursor lesions could provide evidence that an accumulation of genetic events is required in order for normal ovarian epithelium to generate benign, borderline, malignant tumors. Few pre-invasive ovarian tumors have so far been studied. 30 cases of ovarian epithelial tumor, including benign, borderline, and malignant tumors, were analyzed for microsatellite instability (MSI) by gel analysis of paired germ line and tumor DNA. PCR amplification was performed using the panel of 5 microsatellite markers recommened by the NCI (BAT25, BAT26, D2S123, D5S346, D17S250) and 6 additional markers (D1S160, D1S162, D7S522, D11S860, D17S855, D17S932). In this study, D2S123 and D5S346 were the most frequently altered markers in malignant ovarian tumors and D11S860 locus showed MSI in 2 adenomas, 2 boderline tomors, and 6 malignant tumors. Other markers displayed instability with only one or two tumors showing MSI. On the basis of NCI criteria, most benign tumors demonstrated microsatellite stable (MSS). In the borderline tumors, 5 tumors revealed MSS, 4 tumors had low-frequency MSI (MSI-L), and one tumor had high frequency MSI (MSI-H). In the malignant tumors, 3 tumors revealed MSS, one tumor had MSI-L, and 6 tumors had high MSI-H. According to the result, MSL-H is more frequently seen in the malignant tumors. D2S123 and D5S346 were the most frequently altered markers in the malignant tumors, and D11S860 locus may be involved in early step of carcinogenesis.