Identification of turnip mosaic virus isolated from Wasabia Koreana Nakai : 고추냉이에서 분리한 turnip mosaic virus 분리동정
The Graduate School of Kyungpook National University
Department of Agricultural Biology
고추냉이 Agricultural Biology Wasabia Koreana Nakai 농생물학;
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Wasabi (Wasabia Koreana Nakai) leaves showing severe mosaic symptom were collected from the isolated field of Yeongnam Agricultural Research Institute in Kyungnam province. The results of indicator plant tests by infected leaves showed necrotic local lesions on inoculated leaves of Chenopodium amaranticolor, C. quinoa, Gomphrena glubosa, Nicotiana tabacum cv. Samsun, N. tabacum cv. KY-57, N. tabacum cv. Turkish, N. tabacum cv. Xanthi-NC, N. tabacum cv. Xanthi-NN, N. tabacum cv. White Barley and Tetragonia expensa while, Nicotiana occidentales, Physalis floridana and Raphanus sativus showed systemic infection. Under electron microscopy, filamentous particles of 720×12 nm in diameter, and the typical inclusion bodies such as pinwheel, scroll and laminate shapes were found in preparations of the infected leaves. The virus was identified to be to Turnip mosaic potyvirus (TuMV) by its host range test, particle morphology, enzyme-linked immunosorbant assay (ELISA). For detection of the virus, reverse transcription and polymerase chain reation (RT-PCR) was performed with a set of 22-mer TuMV-specific primers to amplify a 986 bp DNA fragment. This DNA fragment included the whole coat protein (CP) gene. To investigate homology difference of the CP region by each host with TuMV isolate from radish which is reported previously, we sequenced this region. By sequence analysis of the CP gene, we could detect that it was consisted of 864 nucleotides and 288 amino acids. As compared this region with TuMV isolate of radish, it showed about 90.2% nucleotide sequence homology and 96.2% amino acid sequence similarity.