삽살개의 비색에 따른 TYRP1 염기설열 비교와 STR marker를 이용한 모색집단의 유전적 근연관계 분석
TYPR1 sequence comparison and Phylogenetic analysis of brown nose Sapsaree among different coat color groups of Sapsaree
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We studied Tyrosine related protein 1 (TYRP1) sequence comparison and phylogenetic analysis of brown nose Sapsaree among different coat color groups of Sapsaree. We classified Sapsaree population which contains 471 adult dogs into 3 categories according to nose color pattern; Black (459), Brown (10), Self (2). All of brown nose Sapsaree dogs have yellow coat color and all of self nose Sapsaree dogs have white coat color. For TYRP1 sequence analysis we genotyped 4 groups of black nose color dogs (Sapsaree (6), Jindo (2), English Coker spaniel (1)), brown nose color dogs (Sapsaree (10), English Coker spaniel (1), Labrador Retriver (1), Siberian Husky (2), English Pointer (1)), self nose color dogs (Sapsaree (4)), red nose color dogs (Jindo (3), Pungsan (2)). We identified four relatively common DNA sequence variants in brown and self nose color Sapsaree dogs in TYRP1 sequence. Three DNA sequence variants (C41S, F58S, P80P) were found in exon 2. The other variant contained a premature stop codon in exon 5 (Q331X). All black nose color dogs contained neither of exon 2 or exon 5 DNA sequence variants. All 10 of the brown nose color Sapsaree dogs carried exon 2 or exon 5 of these sequence variants likely to interfere with TYRP1 function. White Sapsaree dogs which were DNA variants in exon 2, had self colored nose and which were not, had black nose. Difference mutation of TYRP1 gene mutations effect on the nose color of Sapsaree. Mutation of TYRP1 gene which were found in Sapsaree was not identified in Jindo and Pungsan dogs. So nose decolorization of Jindo and Pungsan dogs was supposed to be mediated by different mechanism from those of Sapsaree. Brown nose color of western dog breeds was supposed to be determined by similar mechanism of pigment biosynthesis and deposition. We prepared samples from 3 groups of brown nose Sapsaree dogs (10), yellow Sapsaree dogs (20) and black Sapsaree dogs (20) with black nose for phylogenetic analysis. As a genetic marker, 7 STR (microsatellite loci) were used. Average values of allele number, heterozygosity and PIC value of the Sapsaree groups were 5.29, 0.765, 0.682 of brown nose Sapsaree dogs; 5.14, 0.753, 0.690 of yellow Sapsaree dogs; 5.43, 0.769, 0.710 of black Sapsaree dogs. In the unweighted pair group method with arithmetic mean (UPGMA) dendrogram based on Nei's DA distance, each brown nose Sapsaree is more closed to yellow coat color group of Sapsaree. It is clear that much addition work will be required before a complete understanding of this results occur. So, we propose an phylogenetic analysis of nose and coat color population using many microsatellite markers in Sapsaree.