재구성 지방에 의해 조절되는 유전자의 탐색 및 동정
Screening and characterization of genes modulated by structured lipid
ii, 66 p.
재구성 지방 지방 대사 지방세포;
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Structured lipids (SLs) are made from vegetable oils. They are processed to include a higher concentration of diacylglycerol (DG). Composition of the SLs is different from natural oils, which are primarily made of triacylglycerol (TG). The potential health benefits associated with SLs include enhancing dietary fat metabolism lowering blood lipids and controlling body fat. Ingestion of DG with fatty acids predominantly located at the 1,3 positions on the glycerol backbone appears to bypass traditional pathways of fat re-synthesis, transport and storage. The 1,3-DG are converted into 1- or 3-monoacylglycerol (MG) by lipases in the small intestine, but do not get repacked into TG and chyromicrons, instead get diverted into the portal vein to the liver. In the liver, the fatty acids are fed into an extensive lipid oxidation, which results in the breakdown of lipids, instead of storage or synthesis in the case of triglycerides. Since the SLs has been to be such an effective molecules in the lipid metabolism, it would be interesting to search genes affected by the SLs in the cell culture system. In this study, in order to identify genes differentially expressed by SLs, we performed a technique called differential display reverse transcriptase polymerase chain reaction (DDRT-PCR) and microarray analysis, after cells (3T3-L1 and HepG2) were treated with SLs. All 80 of differentially expressed cDNA bands, including PPAR and , which are known as major regulators in the lipid metabolism, were selected in the DDRT-PCR. Changes in their expression were confirmed by Northern blot analysis. In microarray analysis, numerous genes were found to be differentially expressed, which are related to lipid metabolism and cell differentiation. Among those genes, expressional patterns of the PPAR and PPAR genes in response to the SLs were of a special interest, since they have been acknowledged as key regulators in the lipid metabolism. The level of PPAR expression was shown to be increased as the SLs was added to the culture media, while that of PPAR was decreased, suggesting a pivotal role of the SLs in the lipid metabolism. In search of genes affected by the PPAR family, the expression of genes related to the lipid degradation (L-FABP, UCP-2, FACO etc.) were increased, while those related to the lipid synthesis and accumulation (LPL, C/EBP) were decreased, Further studies are necessary to persue the results of this study in order to understand the detailed role of SL in lipid metabolism.