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용균성 야생 점액세균의 분리와 특성 조사 원문보기
Isolation and Characterization of Bacteriolytic Wild Myxobacteria

  • 저자

    박수연

  • 학위수여기관

    호서대학교 대학원

  • 학위구분

    국내석사

  • 학과

    생화학과 미생물분자유전학 전공

  • 지도교수

  • 발행년도

    2003

  • 총페이지

    iii, 52p.

  • 키워드

    용균성 야생 점액세균;

  • 언어

    kor

  • 원문 URL

    http://www.riss.kr/link?id=T10050844&outLink=K  

  • 초록

    Myxobacteria are Gram-negative soil bacteria that display a complex life cycle and social behaviors, including fruiting body development. On a nutrient-rich surface, rod-shaped cells grow vegetatively. However in a nutrient-depleted environment, swarms of about 100,000 cells move into on aggregation center and form multicellular fruiting bodies. In addition myxobacteria are known to be one of the last microbial resources for finding of valuable bioactive compounds and enzymes. However only a fraction of wild myxobacterial strains have been isolated until now and a major portion of wild myxobacteria in nature are remained to be isolated because the handling of this organism is not easy and the practical value of myxobacteria have been known recently. Thus, as an effort to collect these valuable biological resources, our lab isolated 204 wild myxobacterial strains from nature. In this study, I determined partial 16S rRNA sequences of all myxobacterial isolates and divided the 204 isolates as 7 groups based on their relatedness. 4 isolates identified as Stigmatella spp. constituted Group 1, 5 isolates identified as Archangium spp. belonged Group 2, 49 isolates identified as Mycococcus coralloides constituted Group 3, 41 isolates identified as Myxococcus stipitatus belonged to Group 4, 21 isolates identified as Myxococcus fulvus belonged to Group 5, 24 isolates identified as Myxoxococcus macrosporus belonged to Group 6, 60 isolates identified as Corallococcus exiguus belonged to Group 7 and divided into 7 groups. Most isolates showed same cellular morphology, long rod-shaped vegetative cells and spherical myxospores. However isolates of each group formed unique fruiting bodies that are different from those of other groups. It has appeared that locations and properties of soil samples have not much effects on the types of isolates. It also has been implied that although the isolates in the same group are closely related based on the 16S rRNA sequences, they may not be same strains if they are isolated in different locations. When 10 isolates that were belonged to Group 7 but isolated in different locations were placed on CY plates, each of isolates showed different shapes of colony edges. In addition, I isolated 6 halophilic myxobacteria from costal soils, which were identified as Myxococcus fulvus, Myxococcus virescens, Myxococcus macrosporus and Myxococcus xanthus.


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