Carbonic anhydrase의 산화-환원 조절 가능성
Possible redox-regulation of carbonic anhydrase
산화 환원 응용생명과학 단백질정량 단백질정제;
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Carbonic anhydrase (CA) is an enzyme catalyzing reversible hydration of CO_(2). It was shown recently that CA from tobacco binds to salicylic acid and involves in plant defense reaction. Our previous data showed that in vivo CA of A. thaliana contains disulfide bond, suggesting that its activity might be controlled by cellular redox condition. It was also known that NCE103, a carbonic anhydrase homolog of S.cerevisiae is essential for aerobic growth of yeast. Here we show that CA from A. thaliana is able to support aerobic growth of NCE103 mutant. We generated five Cys □ Ser mutants of Arabidopsis CA and found that two of them failed to support aerobic growth. We purified chloroplast carbonic anhydrase and showed that in oxidation conditions it could form multimers linked by intermolecular disulfide bond. Possible control of carbonic anhydrase by reversible disulfide bond formation is presented.