NaCI Salt stress에 대한 수박 접목 유묘의 생리적 반응
Physiological responses of grafted watermelon seedling (Citrullus vulgaris schrad.) to NaCI stress
NaCI stress 수박접목유묘 원예학;
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Seedlings of 'Sambok Honey Watermelon' grafted on 'FR-Dantos' bottle gourd were used to monitor physiological responses and the changes in protein species in the presence of NaCl. Grafted watermelon seedlings were treated with 0, 45, and 90 mM NaCl for up to six days. It was hoped that the results of this study should provide valuable information for further studies aimed to understand salt tolerance mechanism of watermelon. Seedling growth as well as the concentrations of total proteins and nitrogen decreased in the presence of NaCl.; the higher the NaCl and the longer its presence, the more they decreased. In the presence of NaCl, the concentrations of Na and K increased in the leaves, but that of K decreased in the roots. Also increased included malondialdehyde (MDA), a product of lipid peroxidation. proline, time zero concentration being higher in the leaves than in the roots, increased with NaCl. Such an increase in proline was greater in the leaves. Activities of superoxide dismutase (SOD), ascorbate peroxidase (AP), catalase (CAT), and gluthathione reductase (GR) increased with increasing NaCl concentrations, and a greater increase was observed in the roots than in the leaves. In seedling leaves, two proteins at 45 mM and five proteins at 90 mM NaCl newly appeared. However, two new proteins appeared at 45 mM disappeared at 90 mM NaCl. Electrophoretic separation of proteins and matrix assisted laser desorption time of flight (MALDI-TOF) analysis indicated that a new protein appeared at 90 mM NaCl was found to be the same as flavonoid-3'-hydroxylase reported in rice. In the roots, four proteins increased and eight proteins appeared at 45 and 90 mM. Eighteen proteins increased and eleven proteins appeared at 90 mM. From the MALDI-TOF analysis, six proteins were identified in the roots.: one increased at 45 and 90 mM, one appeared at 45 mM and four increased only at 90 mM NaCl. Such changes in proline concentrations, lipid peroxidation, the activities of the antioxidant enzymes related to scavenge reactive oxygen species, and the appearance and disappearance of protein species should have some implications in salt tolerance of watermelon. Further studies are needed to relate these changes to the difference in salt tolerance of scion cultivars and various rootstocks of watermelon.