건선 치료제인 Retinoic acid 의 혈관형성 억제작용
Inhibition of Angiogenesis by Retinoic Acid, a Therapeutic Agent for Posriasis
건선치료제 혈관형성 Retinoic acid;
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Background : Psoriasis is a well known disorder of keratinization. In this disease, several reports revealed that dermal micro vesselsand vascular endothelial cell growth factor (VEGF) are increased or over-expressed. Angiogenesis may play an important role in progression of psoriasis. Retinoic acid(RA) is widely used as an anti-psoriatic drug because of its potent action on keratinocyte growth and differentiation. But, the effect and precise mechanism of RA on angiogenesis are uncertain. Objective : The goal of this study was to investigate the effects and action mechanisms of RA on angiogenesis induced by VEGF. Materials & Methods : In vitro studies were performed using human umbilical vein endothelial cells (HUVEC) and human dermal micro vascular endothelial cells (HDMEC) cultures. After inducing angiogenesis by VEGF on those cultured cells, I analyzed dose-dependent changes before- and after- all-trans retinoic acid (Sigma, USA) treatment. Angiogenesis was observed according to the stages; endothelial proliferation, migration, and tube formation. In vivo studies, using Chorioallantoic membrane (CAM) assay with or without VECF induction, was done to check developmental effect on embryo vasculature. Results : RA had no effects on endothelial proliferation. However, RA showed statistically significant inhibitions on endothelial migration and tube formation, in dose-dependent manner. RA also inhibited embryological vascular development. Conclusion : RA revealed inhibitory effects on angiogenesis in vivo and vitro study. Further therapeutic approaches to psoriasis could be possible on the aspect of angiogenesis.