참죽나무에서 분리한 Flavonol glycosides의 암전이 억제 활성
Antimetastatic activity against cancer cell of flavonol glycosides isolated from cedrela sinensis A. Juss
참죽나무 쌍자엽식물 암전이 응용생명과학 식물학;
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The majority of cancer patients who succumb to their disease die from metastasis. Metastasis is a sequential process in which tumor cells detach from the primary growth, invade through the surrounding host tissue into the circulation and subsequently disseminate to distant organs, where they arrest, extravasate and proliferate to from metastatic foci. The events that lead to the metastasis are (a) detachment from the primary tumor, (b) circulation through blood and lymphatics, (c) adhesion to the extracellular matrix, (d) invasion after the proteolytic cleavage of the basement membrane, and (e) adhesion and new growth at the new site. If one of the these events could be inhibited it can lead to the arrest of metastatic growth. During invasion and metastasis, tumor cells must adhere to and penetrate the basement membrane and interstitial stroma. This process clearly involves the specific interactions between tumor cell surface adhesion molecules and components of extracellular matrix. Highly invasive tendencies of tumor cells to extracellular matrix were associated with the increased production of matrix metalloproteinases. It was known that augmented levels of metalloproteinases in tumors correlated with the developments of invasion and metastasis of the human breast, colon, stomach, thyroid, lung and liver cancer. Type Ⅳ collagenase, which degrades type Ⅳ collagen, the structural backbone of the basement membrane barrier, is a very promising target enzyme for screening antimetastatic agents. This study was to find flavonoid derivatives as Type Ⅳ collagenase inhibitor from Cedrela sinensis A. Juss. Flavonoid derivatives were isolated by activity guided fractionation and characterized from thier spectroscopic data (NMR, IR, Mass). The biological activity of these compounds were tested by using inhibitory activity of matrix metalloproteinase-2 (Type Ⅳ collagenase) method together with a cytotoxicity and apoptosis test against human cancer cell lines.