느타리버섯균사체 액체배양물 유래 이소플라본 다당체의 마우스에 대한 에탄올대사 증신 효과
Effects of isoflavone-linked polysacchrides from submerged-liquid of pleuotus ostreatus on the enhancement of ethanol metabolism in mouse
느타리버섯 버섯균사체 이소플라본다당체 에탄올대사;
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Influence of extract from the submerged-liquid culture of Pluorotus ostreatus (PO) on the reduction of blood alcohol level in mouse, purposely intubated alcohol, was studied, and from which active compounds were isolated. Crude extract (4.8 mg) from several liquid cultures of mushrooms, including PO, were intubated, p.o., into mice, 10 min after alcohol intubation (5 g/kg), resulted in the reduction of blood alcohol concentration; among the extracts, PO extract showed a best activity (53.5% reduction). The reduction activity of PO extract was a proportional to doses of treatments; 87.4% reduction by 7.2 mg PO extract. PO extract(17.5g solid materials/ℓ) contained 887 mg/ℓ total protein, 205 mg/ℓ total lipid, and 13.1 g/ℓ total sugar including 930 mg/ℓ b-D-glucan. It contained genestein and diadzein, 1.17 and 3.06 mg/ℓ, respectively, as minor constituents. PO extract was fractionated and the blood alcohol reduction of its fraction was examined. First, hexane- and chloroform-soluble materials were removed and the remained residue, called ICP, was treated with 80% ethanol to give ICP80. ICP80 was further purified by DEAE-Cellulose chromatography, ICP80D, followed by silica gel chromatography, to give 1CPS. Blood alcohol reduction in mice by 4.8 mg of ICP, ICP80, ICPD and ICPS was 77.9, 75.7, 80.7, and 85.2%, respectively, and also by 50 ug of genestein and diazdein was 71.7 and 68.1%, respectively. In vitro experiments, all these samples were enhanced activity of ADH; especially, the activity of 3 mg each of ICPS, ICPD and ICPS was 289, 319, and 324%, relative to that of control, PO and ICP. ADH activity of the mouse live homogenate from mouse treated with 4.8 mg of PO, ICP, ICP80, ICPD, and ICPS showed 127, 124, 134 and 150%, respectively. ADH activity (135%) of mouse liver homogenate was also enhanced by 50 ug diadzein. Nitric oxide (NO) concentration in blood of mice treated with alcohol was increased by a dose dependent manner, which was concommetant with the elevation of NO concentration. In contrast, by the inhibitor treatment of iNOS resulted in the elevation of alcohol concentration, but not NO concentration. ICPS treatment reduced NO production, but increased the activity of ADH by a dose-dependent manner. Active materials in ICPS was found to be genestein-O-β-D-glucan and/or diadzein-O-β-D-glucan when analyzed by TLC, HPLC (BioSep 2000), IR, UV/VIS and NMR spectrosophy. Estimated molecular weight of these materials were 18,000 daltons. The blood alcohol-reducing activity of PO extract also shown in human trials by ranging from 17.2% to 45.8%. In conclusion, PO extract reduced blood alcohol concentration in animal and human trials. The active fraction contained isoflavone-O-b-D-glucan (mw, 18,000) with a composition of glucose:arabinose:galctose:diazdein (98:1:1:1, w/w/w/w). This compound significantly reduced NO production in mouse.