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Hemin-agarose 친화크로마토그래피로 분리한 Helicobacter pylori 26695의 프로테옴 분석 원문보기
Enrichments of helicobacter pylori strain 26695 protein using hemin-agarose affinity chromatography

  • 저자

    박희진

  • 학위수여기관

    慶尙大學校 大學院

  • 학위구분

    국내석사

  • 학과

    신경생물학과

  • 지도교수

  • 발행년도

    2004

  • 총페이지

    iii, 32p.

  • 키워드

    HELICOBACTER PYLORI HEMIN-AGAROSE 크로마토그래피 프로테옴 미생물학;

  • 언어

    kor

  • 원문 URL

    http://www.riss.kr/link?id=T10060097&outLink=K  

  • 초록

    Helicobacter pylori is a well-known human pathogen that causes gastritis, gastroduodenal ulcers, and gastric cancers. The soluble protein of H. pylori strain 26695 was extracted and fractionated on hemin-agarose resin. The hemin-binding fraction was collected and analyzed by 2-dimensional electrophoresis. The2-DE-PAGE-displayed spots after silver staining, were excised and identified by matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF-MS). Approximately, the 170 protein spots processed and 94proteins were identified. The identified spots represented 58genes. Thirty-four proteins were newly identified in this study, including fumarate reductase, iron-sufur subunit (FrdB). Of them, proteins involved in replication, recombination, repair, transcription, signal transduction, cell motility, energy production, and lipid metabolism were highly enriched by this method. The results of this study shows that low-abundant proteins of H. pylori are efficiently concentrated by using affinity resin and analyzed by analyz.


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