Hemin-agarose 친화크로마토그래피로 분리한 Helicobacter pylori 26695의 프로테옴 분석
Enrichments of helicobacter pylori strain 26695 protein using hemin-agarose affinity chromatography
HELICOBACTER PYLORI HEMIN-AGAROSE 크로마토그래피 프로테옴 미생물학;
- 원문 URL
Helicobacter pylori is a well-known human pathogen that causes gastritis, gastroduodenal ulcers, and gastric cancers. The soluble protein of H. pylori strain 26695 was extracted and fractionated on hemin-agarose resin. The hemin-binding fraction was collected and analyzed by 2-dimensional electrophoresis. The2-DE-PAGE-displayed spots after silver staining, were excised and identified by matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF-MS). Approximately, the 170 protein spots processed and 94proteins were identified. The identified spots represented 58genes. Thirty-four proteins were newly identified in this study, including fumarate reductase, iron-sufur subunit (FrdB). Of them, proteins involved in replication, recombination, repair, transcription, signal transduction, cell motility, energy production, and lipid metabolism were highly enriched by this method. The results of this study shows that low-abundant proteins of H. pylori are efficiently concentrated by using affinity resin and analyzed by analyz.