미더덕 껍질로부터 Glycosaminoglycans의 추출 및 분획
Fractionation of glycosaminoglycans extracted from the styela clava tunic
미더덕 GLYCOSAMINOGLYCANS 수산과학 생물자원;
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Proteoglycans combined glycosaminoglycans(GAGs) with protein. These molecules containing a repeating disaccharide unit wear included amino sugar such as N-acetylgalactosamine (GalNAc) or N-acetylglucosamine (GlcNAc) and uronic acid (glucuronic acid or iduronic acid). Optimum extracting condition of glycosaminoglycans from Styela clava tunic was first extract method at 105℃ heat in ion water containing 1/60 M sodium phosphate dibasic. Trichloroacetic acid(TCA), hydrochloride(HC1) and 5-sulfosalicylic acid(SSA) were used for deprotein of the GAGs. Concentration for deprotein of GAGs were 5.0% TCA(w/v) and 10.0% HC1(v/v) treatment. The sulfate and protein contents of deproteinized GAGs contained 35.1% and 22.0% for TCA(w/v) and 35.4% and 18.5% for HC1, respectively. Characterization of extracted crude glycosaminoglycans had similar component, amino acids, composition sugars, mineral contents compared with Dermatan sulfate from mammalia. Extracted glycosaminoglycans from Styela clava tunic had similar content rate of Carbon, Hydrogen, Nitrogen and Sulfur element compared with Dermatan sulfate and Chondroitin-6-sulfate. Crude glycosaminoglycans were fractionated two peaks by ion exchange chromatography on DEAE-sepharose CL-6B.