버섯액체배양 추출물의 항산화 증진 효과
Antioxidant activity of hot-water extracts from submerged-liquid cultures of mushrooms containing mulberry tree powder
버섯액체배양 버섯작물 항산화 Antioxidant activity submerged-liquid cultures of mushroom;
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Effect of mulberry tree powders on the antioxidant activity of submerged-liquid culture of mushrooms was investigated. Agaricus blazei (AB, Shinryeong), Pleurotus ostreatus (PO, Neutari), Hericicum erinacium (HE, Norugungdengyee), Phellinus linteus (PL, Sanghwang) and Paecilomyces japonicus (PJ, Dongchunghacho) were cultured in a shaking incubator (200 rpm, 25℃) for 7 days in culture media: 1) basal medium (BM), 2) BM+1% mulberry tree powders (BMM), 3) BM+1% pine tree powders (BPM) and 4) BM+0.5% mulberry powders+0.5% pine tree powders (BMPM). The antioxidant activity of the hot-water extracts of the cultures were measured in systems of autooxidation and free-radical scavenging systems. Antioxidant activity in autooxidation system : Each 1 mg of freeze-dried hot water extracts of those cultures was reacted with mixture of 10 mL of 0.2 M sodium phosphate buffer (pH 8.0), 4.5 mL of distilled water and 10.5 mL ethanol containing 275 μmol linoleic acid, and incubated (200 rpm, 40℃) for 16 days. Peroxide value (POV) was measured for a period of over 16 days, and malonaldehyde (MA) was determined only for samples from the day 16 of incubation. The antioxidant activities of AB-cultured in BMM (AB-BMM) and HE-cultured in BMM (HE-BMM) were superior to those of other mushroom strains-cultured in BMM or BM and of BMM. These results suggest that mulberry tree powders enhance the antioxidant activity of submerged-liquid culture of mushroom strains. The AB-BMM and HE-BMM were the most active cultures. Antioxidant activity of free-radical scavenging system: Freeze-dried hot water extracts (designated crude extracts) were fractionated with hexane, chloroform, ethylacetate, and butanol. Antioxidant activity of each sample was examined by the Fenton's reagent-induced linoleic acid oxidation system and mouse liver microsomal oxidation system. In Fenton's reagent oxidation system, crude extracts from the cultures of PO-BMM, PL-BMM and PJ-BMM exhibited stronger antioxidant activity than any other fractions (hexane, chloroform, ethylacetate, butanol and aqueous fractions). Of the crude extracts, the crude extract from PO-BMM culture was stronger than other crude extracts. These results observed in Fenton's reagent system was also seen in the mouse liver microsomal system. In conclusion, crude extracts of PO-BMM could be useful extracts for functional materials to reduce the oxidation of lipids in food systems induced by autooxidation, whereas crude extracts of PO-BMM, PL-BMM, and PJ-BMM cultures, especially PO-BMM, could be useful extracts for functional materials to reduce the oxidation of lipids in food systems induced by free radicals. Further research in necessary to elucidate active materials to give some information for the functionality of the extracts.
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