Trichoderma harzianum YC459에 의한 잿빛곰팡이병의 생물학적 방제와 기주식물과의 상호작용
Biological control of gray mold rot by trichoderma harzianum YC459 and its interaction with host plantsco
잿빛곰팡이병 생물학적방제 기주식물 미생물학 응용생명과학;
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Efficacy of biocontrol agent, Trichoderma harzianum YC459(TORY^(�)) for the control of gray mold rot of vegetables caused by Botrytis cinerea was evaluated in growth chamber tests or commercial greenhouses. In three greenhouse testes using tomato at Jinju, Sunchun and Cheongju, T. harzianum YC459 showed the control value of 45%, 73% and 66%, in comparison to that of chemical fungicide, Dichlone, 56%, 88%, 90%, respectively. Those efficacies were not significantly different from chemical fungicides, although the mean values of chemical fungicides were a little bit higher than those of T. harzianum YC459. The growth of hot pepper (Capsicum annuum L.) seedlings was also promoted by the 1% mixture (weight) of T. harzianum YC459 granules in nursery container mix during 2-month cultivation at a commercial farm. The growth of young radish (Raphanus sativus L.) was also significantly increased by the soil treatment of 1kg granules on the soil surface of 165㎡. During experiments in commercial greenhouses, senescent flower petals or flowers of vegetables such as tomato, strawberry, hot pepper and zucchini squash were blighted to be removed from fruits within three or five days after spraying of T. harzianum YC459. The mechanism for selective colonization of senescent floral tissues by T. harzianum YC459 was elucidated using fresh and senescent (3 days and 12 days after manual pollination, respectively) floral tissues of zucchini squash (Cucurbita moschata Duchesne). The spores of T. harzianum YC459 were produced more on agar and liquid culture media supplemented with 5% dry powder of senescent floral tissues than with fresh tissues during 15 days incubation at 28℃. Mycelial growth was also much better in the media supplemented with senescent tissues than with fresh tissues. Enzyme activities of cellulase, amylase, and polygalacturonase in the liquid media, which might be involved in the colonization of tissues by T. harzianum YC459 were compared. The activities of three enzymes were much higher in the media with senescent floral tissues than with fresh floral tissues reaching to the maximum during 9 to 12 days of incubation. Based on the results, the selective removal of senescent floral tissues, a possible inoculum source of the pathogen, may be another mechanism for biocontrol of gray mold rot of vegetables by T. harzianum YC459.