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크로마핀 세포가 분비하는 Catecholamine 생체 내·외 정량분석 원문보기

  • 저자

    박준모

  • 학위수여기관

    慶北大學校

  • 학위구분

    국내석사

  • 학과

    의학과 마취과학전공

  • 지도교수

  • 발행년도

    2005

  • 총페이지

    23, ii p.

  • 키워드

    마취과학;크로마핀 세포;Catecholamie;

  • 언어

    kor

  • 원문 URL

    http://www.riss.kr/link?id=T10062287&outLink=K  

  • 초록

    Adrenal medullary transplants into the spinal cord subarachnoid space have been demonstrated to reduce pain sensitivity. This analgesia most likely results from the release of neuroactive substances, particularly catecholamines and opioid peptides from the transplanted cells into the CSF of the spinal cord. In our laboratory, chromaffin cells were encapsulated with alginate not to be bothered by immune reaction. Catecholamines and met-enkephalin from encapsulated chromaffin cells were measured quantitatively in vitro. The neurotransmitters were also measured more directly from adrenal medullary transplants in the spinal cord CSF. Isolated bovine chromaffin cells were encapsulated with alginate and poly-L-lysine prior to implantation into rat subarachnoid space to protect them from host immune system. And then catecholamines from encapsulated chromaffin cells were measured quantitatively in vitro by HPLC. The animals were randomized into 2 groups, one of which received microencapsulated chromaffin cells and the other empty capsules. The effects of such implants were evaluated on the pain behavior resulting from a chronic constriction injury of the rat sciatic nerve for 30 days. Catecholamine concentration in CSF was analyzed. Data (mean SD) are considered significant at a P


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