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  • 저자

    현 승훈

  • 학위수여기관

    慶北大學校

  • 학위구분

    국내석사

  • 학과

    화학공학과 화학공학전공

  • 지도교수

  • 발행년도

    2005

  • 총페이지

    ix, 96 p.

  • 키워드

    박테리아 셀룰로오스;

  • 언어

    kor

  • 원문 URL

    http://www.riss.kr/link?id=T10062295&outLink=K  

  • 초록

    It is well known that cellulose-producing (Cel^(+)) cells are converted to non-cellulose producing (Cel^(-)) mutants in a shear stress field such as agitated culture. The conversion of Cel^(+) cells from Gluconacetobacter hansenii PJK (KCTC 10505 BP) and Cel^(+) cells from the agitated culture to Cel^(-) mutants was investigated by measuring the colony forming unit (CFU). This was achieved in a shaking flask with three slanted baffles, which exerted a strong shear stress. Substituting glutamic acid for acetic acid accelerated the conversion of microbial cells from a wild type to Cel^(-) mutants and decreased BC production in a conventional flask, but depressed the conversion of microbial cells from a wild type to Cel^(-) mutants and increased BC production in a baffled flask. Substituting glutamic acid for acetic acid accelerated the conversion of Cel^(+) cells from the agitated culture to Cel^(-) mutants and decreased BC production in a conventional flask but decreased BC production in a baffled flask although the conversion of Cel^(+) cells from the agitated culture to Cel^(-) mutants decreased. The supplementation of 1% ethanol to the medium containing organic acid depressed the conversion of microbial cells from a wild type and Cel^(+) cells from the agitated culture to Cel^(-) mutants and increased BC production, but accelerated the conversion of microbial cells from a wild type and Cel^(+) cells from the agitated culture to Cel^(-) mutants and decreased BC production using the medium containing glutamic acid in a baffled flask. Waste of beer fermentation broth was worthy of a substitute for conventional medium for the producing of BC in a shaking culture because that is an inexpensive carbon, nitrogen source with ethanol. Waste of beer fermentation broth contained more C and N than a basal medium and contains a little S and more than 4% ethanol. The production of BC decreased in a shear stress filed like a shaking culture in a baffled flask and an agitated culture in a jar fermentor. The Cel^(-) mutants converted from Cel^(+) cells decreased as the impeller speed increased. There was an optimum impeller speed in maximizing the number of Cel^(+) cells and BC production.


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