효율적인 암 유전자치료를 위한 종양세포 표적 아데노바이러스 벡터의 개발
Development of the cancer cell targetadenovirus vector for the efficient cancer gene therapy
암 유전자치료 아데노바이러스;
- 원문 URL
Adenoviral vectors have been widely used for cancer gene therapy. Adenovirus entry is dependent upon expression of coxsackie and adenovirus receptor(CAR). However, CAR is sometimes not present on surfaces of cancer cells. Because of these limited adenovirus entry, fiber-modified adenovirus vectors capable of entering cells via specific receptors other than CAR would be highly useful for delivering genes into specific cell types. Fiber-modified adenoviral vector by adding of small peptide ligand was tested for the delivery of a transgene to cancer cells. The fiber-modified adenovirus can mediate entry and expression of a β-galactosidase reporter gene into cancer cells more efficiently compared the unmodified adenovirus. Particularly, the gene transfer efficiency was improved up to 5 times in OVCAR3 cells, a ovarian cancer cell line. Such gene transfer systems hold promising for delivering genes to transferrin receptor overexpressing cancer cells, and could be used for future cancer gene therapy.