Identification, Expression and Application of SAM Synthetase Genes from Streptomyces Peucetius var. Caesius ATCC 27952
Hang Thi Tong
The Graduate School of Sunmoon University
Department of Chemistry Biochemistry Major
Streptomyces Peucetius var. Caesius Identification ATCC 27952 Application Expression SAM Synthetase Genes;
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Streptomyces peucetius is only one organism known to produce doxorubicin - the antibiotic with anticancer activities, and so far, only daunorubicin/doxorubicin were produced in this strain. Genome of Streptomyces peucetius var. caesius ATCC 27952 has been sequenced for the first time in our lab. From this sequence data, many elements those show the effect on the regulation of whole system as well as on the production of secondary metabolites were found and analysed. Among these elements, S-adenosyl-L-methionine (SAM) has caused the visible change in the morphological differentiation seen in some strains. Moreover, it also results in the antibiotic production change when its expression level in the cell varies. The formation of SAM is catalysed by enzyme S-adenosyl-L-methionine synthetase (or Adenosine 5'-triphosphate: L-methionine S-adenosyltransferase). In whole genome sequence, two open reading frames encoded for this enzyme were found to share the high identity (> 88%) with the similar ones in S. avermitilis and S. coelicolor. From this information, we decided to characterize SAM synthetase genes in this strain and see its effect on the either morphological or antibiotic production changes in other Streptomyces spp. This dissertation is devided into four chapters. The general introduction is delineated in chapter 1. Chapter 2 describes in detail the materials and methods used in this study. Results, discussions and conclusion will be explained in next following chapters.