Recombinant antibody-toxin with Fab2(GPQ) domain
박테리아균주 단백질 Plasmids;
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Recombinant antibody-toxins which are used as anticancer therapeutics are genetically engineered fusion protein of a tumor specific antibody and a toxin from bacteria or plants. The curved antibody-toxin [B3(Ab1)-PE38]_(2) is a body form of slim antibody-toxin B3(Ab1)-PE38. B3(Ab1)-PE38 is composed of Ab1 domain of monoclonal antibody B3 and PE38, a truncated form of Pseudomonas exotoxin(PE). Monoclonal antibody B3 is a murine antibody targeted to the Lewis^(Y)-related carbohydrate antigens that are abundant on the surface of many tumor cells. In previous study, we confirmed that curved antibody-toxin has higher cytotoxicity than slim antibody-toxin. This result indicates that the higher shape of antibody-toxin increase cytotoxic activity on tumor cell. Based on this result, curved antibody-toxin, [B3(D6)-(AM)_(n)-B3(D6)-PE38]+[B3(D5)] that has left Ab1 domain was constructed. But the refolding yield of this curved antibody-toxin was very low. It was assumed that the refolding of this antibody-toxin was interfered with(AM)_(2), (AM)_(3) peptide. In this study, we made new left curved antibody-toxin via different peptide sequences linking C-terminal and N-terminal of the two Ab1 domains.