활성산소종에 의한 DNA 손상에 대한 상황버섯과 홍삼 추출물의 효과
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The effect of Phellinus linteus and red ginseng on the E, coli ColE1 plasmid DNA break and on the nuclear DNA damage of rat hepatocyte by reactive oxygen species was investigated in this study. The water extract and ethanol extract of Phellinus linteus inhibited the breaking of DNA strand induced by oxidative damage. Water fraction, n-butanol fraction and ethylacetate fraction of red ginseng inhibited DNA strand breaks by oxidative damage, but n-butanol fraction and ethylacetate fraction were more effective than others. It is likely that saponin as well as non-saponin components show an anti-oxidant activity. In reaction mix of Phellinus linteus water extract and red ginseng extract, inhibitory effect of DNA damage was observed. Nuclear DNA damage of rat hepatocyte by hydrogen peroxide was decreased by Phellinus linteus water extract, ethanol extract and red ginseng fractions. The reaction mix of Phellinus linteus water extract and red ginseng extract has synergistic inhibitory effect on nuclear DNA damage. These results suggest that Phellinus linteus water extract, ethanol extract and red ginseng fractions could be effective in cellular defense mechanism in vivo against to bacterial plasmid DNA damage and rat hepatocyte nuclear DNA damage by reactive oxygen species. It can be explained that the extract of natural products could scavenge directly hydrogen peroxide like catalase in the eucaryotic cell in vivo.