컴퓨터 프로그램을 이용한 몽고 저빌 측두골 조직 절편의 3차원적 재구성
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Objectives : To establish the method generating three-dimensional reconstruction of the temporal bone with histologic specimen and computer software, and to evaluate the spacial relationship between cochlea, semicircular canals, and facial nerve in the temporal bone of expehmental animal, mongolian gerbil. Materials and methods : A 5-weeks aged mongolian gerbil (Menones unguiculatus) was used. Temporal bone specimen was processed for histologic study and reference marks were made in each paraffin block to permit accurate alignment for computer aided three-dimensional reconstruction of the tissues. The embedded specimen was horizontally sectioned at 10㎛ interval. Each histology section was digitalized and total of 450 image data were entered to the computer software by tracing each region of interest with digitizer. We reconstructed the 3-dimensiona1 images of the cochlea, semicircular canals, and facial nerve. For the semicular canals, we measured the width and height of each canal and the angles between each other canals. For the cochlea, we measured the diameter of each turn and the volume. For the facial nerve, we measured the length of each segment and the angles between neighboring segments. Results : We successfully reconstructed the 3-dimensional images of cochlea, semicircular canals, and facial nerve. The widths of superior, posterior, and lateral canal were 2.114mm, 1.836mm, and 1.996mm, respectively. The heights of superior, posterior, and lateral canal were 2.373mm, 1.246mm, and 1.502mm, respectively. The angle between superior and posterior canal measured 110.5°. The angles between the superior and lateral, and between the lateral and posterior canals were 98.3° and 94.7°. From apical turn to basal turn, the diameter of each turn of the cochlea were 0.766mm, 1.731mm, 2.074mm, and 2.173mm, respectively. The volume of the cochlea was 3.08㎣. The lengths of the labyrinthine, tympanic, and mastoid segment were 1.006mm, 2.012mm, and 2.460mm. The angle between the labyrinthine and tympanic segment was 121.4°, and the angle between the tympanic and mastoid segment was 154.5°. Conclusion : In our study, we successfully reconstructed the 3-dimensional images of inner ear structures and facial nerve, and quantitative analysis of those structures was accomplished. Applying this 3-dimensional reconstructive technique to disease-induced animal model, we would evaluate the progress of specific disease and the efficacy of treatment more accurately.