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소의 受胎率과 生産性 向上을 위한 Progesterone 측정 및 乳成分 分析의 臨床的 活用 원문보기
Use of progesterone determination and milk component analysis for the improvement of conception rate and productivity in cows

  • 저자

    김철호

  • 학위수여기관

    慶尙大學校 大學院

  • 학위구분

    국내박사

  • 학과

    수의학과

  • 지도교수

  • 발행년도

    2004

  • 총페이지

    xx, 140p.

  • 키워드

    소 수태율 유성분 수의학 프로게스테론;

  • 언어

    kor

  • 원문 URL

    http://www.riss.kr/link?id=T10079892&outLink=K  

  • 초록

    This study is designed to determine early pregnancy diagnosis, the recovery state of ovary function, the judgement and utilization of efficacy of progesterone treatment and to discriminate cows with reproductive disorders and anestrus. The contention of the pioneers that indispensability of progesterone in pregnancy maintenance is a biological law of broad validity for 70 years received only isolated support, based on physiologic rather than chemical data. Progesterone is being until currently performed by radioimmunoassay (RIA) using isotopes by assaying. For the reasons that radioisotopes have many problems of safety in health and environmental pollution, special facilities for its handling and discard, also its short and long halflife, with its inherent limitations with respecting to widespread use, recently in foreign countries radioimmunoassay is being substituted to enzyme immunoassay(EIA) which has higher sensitivity and accuracy in assay without using of radioisotopes. A number of enzyme immunoassays for progesterone have been reported. Most progesterone enzyme immunoassays are used double antibody methods. These methods consume considerable time and reagents because of the requirements for several washing and centrifugation steps involving the reactants. Because of these several factors, we developed an effective enzyme-linked immunosorbent assay(ELISA) system instead of enzyme immunoassay using higher titre of progesterone antibody. The optimum conditions of ELISA system was investigated including antibody titres, bound percent and enzyme conjugate progesterone antibodies did not react upon pregnelone, testosterone, estrone, estridiol-17β, aldosterone, cortisol, corticosterone and 11α-dehydroxyco- rtisone(DOC), but reacted upon only progesterone. The intra and inter-assay coefficient of variation accounted for 4.5%, 6.1∼9.4% when using bovine serum. The assay sensitivity was 0.1 ng/㎖. The assay of progesterone in plasma and skim milk is one of the most useful hormone analyses for monitoring the reproductive state of female mammals. With this in mind, the researcher counted on enzyme-inked immunosorbent assay (ELISA) to measure and analyze the progesterone (P_(4)) concentration of plasma and skim milk, measuring also change in the concentration of blood urea nitrogen(BUN) and milk urea nitrogen (MUN) related to the factor of reproductive disorders caused by the intake of provender for dairy cows and making an analysis of their influences on conception rates and reproductive disorders after artificial insemination(AI). A total of 806 heads of Hanwoo cows(266 heads) and Holstein cows (540 heads) at 50 farms in Southern Gyeongsang province were surveyed, respectively. The results obtained were as follows. ; Conception rate was lower in Holstein cows(50.6%) than in Hanwoo cows(55.1%) at the first fertilization and it was shown same tendency at the second fertilization and factors hampering productivity such as conception delay were higher in Holstein cows(32.6%) than in Hanwoo cows(23.9%). The plasma progesterone concentration of pregnant Hanwoo cows stood at over 5.0 ng/㎖ and that of delivery cows accounted for less than 0.50 ng/㎖, and that of estrus cows stood at less than 0.50 ng/㎖. And bulls accounted for less than 0.3 ng/㎖ in plasma progesterone concentration. In order to diagnose early pregnancy, progesterone concentration of plasma and skim milk was measured at an interval of 2 days for 24 days after artificial insemination. It was shown that pregnant cows were low in progesterone concentration substantially when compared with that of non-pregnant cows at the 18th day after artificial insemination and that there was a great difference in progesterone concentration whether cows become pregnant or non-pregnant. However, there were no significant differences in progesterone of plasma and milk between Hanwoo and Holstein cows. Progesterone concentration of plasma and skim milk in pregnant cows increased (7.1±1.1∼8.2±0.98 ng/㎖) for five months after pregnancy and remained at a constant level before parturition(0.5 ng/㎖). Skim milk progesterone profiles in 68 Holstein cows were determined to monitor postpartum ovarian activity by ELISA. Signs of estrus were observed twice daily and status of the ovaries and uterus were examined every 10 days by rectal palpation. The ovarian activities in postpartum period is classified into five types which were normal(14.7%), cycle delayed(inactive ovary 57.4%), cycle ceased with low progesterone(inactive ovary ; 5.9%, follicular cyst ; 2.9%, 8.8%), cycle ceased with high progesterone (persistent corpus luteum 4.4 %), Acyclicity(inactive ovary ; 11.8%, follicular cyst ; 2.9%, 14.7%). The result of transrectal palpation and measurement of plasma and skim milk progesterone profile using "Two sample test"(Day 0 + Day 10) in 73 anestrous Holstein cows were inactive ovaries 30.1%, follicular cyst 19.2%, luteal cyst 16.5%, persistent corpus luteum 13.7%, and silent heat and error of estrus detection 20.5%, respectively. The "Two sample test" was effectively stilized for the differential diagnosis of ovarian dysfunction, especially silent heat of error of estrus detection, follicular cyst and luteal cyst. It was found that progesterone concentration of plasma in cows having inactive ovaries and follicular cyst stood at less than 1.0 ng/㎖ and that progesterone concentration of plasma in cows with luteal cyst and persistent corpus luteum accounted for higher than 2.0 ng/㎖. Differences were found out between both(p


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