에스트로겐이 眞皮纖維芽細胞의 增殖 및 膠原質合成에 미치는 影響
(The) Effect of estrogen on fibroblast proliferation and collagen synthesis
에스트로겐 진피섬유아세포 교원질합성;
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Estrogen is a sex steroid hormone which is known to be helpful to prevent aging process in various ways. It is frequently used in hormone replacement therapy(HRT). Basically wound healing is likely to have same process on the view of cell proliferation and extracellular matrix formation. However, it is not determined yet wheather estrogen up-regulates or down-regulates wound healing. This study was designed to determine the effect of estrogen on proliferation of human dermal fibroblasts and collagen synthesis in vitro which are most important in wound healing. Fibroblasts were isolated from the dermis of adults and cultivated in the presence of either one of 5 concentrations of conjugated equine estrogen (Premarin^(□), Wyeth-Ayerst Co, Philadelphia, PA, U.S.A ; 0, 0.1, 1, 5, 10㎍/㎖). The fibroblasts were seeded at 4.5 X 10^(3) cell/well in Dulbecco's Modified Eagle's Medium/Ham's F-12 nutrient including 5% fetal bovine serum with either one of five different concentration of CEE in 96-well plates. The cells were incubated for three days. For fibroblast proliferation MTT assay method was used. To measure the production of collagen, the collagen type I carboxy-terminal peptide enzyme immunoassay was carried out. All concentrations of CEE stimulated the proliferation of fibroblasts. The best proliferation and maximum stimulation of collagen synthesis was seen at supplementation of 5㎍/㎖ and 10㎍/㎖ of CEE. The collagen synthesis per cell was also maximal at concentration of 5㎍/㎖ CEE. These results demonstrated that CEE influenced human dermal fibroblast proliferation and collagen synthesis and the CEE concentration was important factor in vitro.