본문 바로가기
HOME> 논문 > 논문 검색상세

학위논문 상세정보

Metabolite composition differences and lipid droplet dynamics analysis in siRNA transfected 3T3-L1 differentiated cell 원문보기

  • 저자

    김희진

  • 학위수여기관

    포항공과대학교 일반대학원

  • 학위구분

    국내석사

  • 학과

    시스템생명공학부

  • 지도교수

  • 발행년도

    2014

  • 총페이지

    67 p.

  • 키워드

  • 언어

    eng

  • 원문 URL

    http://www.riss.kr/link?id=T13533421&outLink=K  

  • 초록

    To understand the connection between the function of lipid droplet controlling genes and phenotype in adipocytes, we used label free imaging technique based on CARS(Coherent anti-Stokes Raman spectroscopy), and combined ToF-SIMS(Time-of-flight secondary ion mass spectrometry) to obtain chemical information for further explanation. 3T3-L1 cells were transfected with four different kinds of siRNAs during differentiation. Abcd2, adrp, kif3a, plin genes were knockdowned temporarily and the effects on lipid droplet formation and dynamics were imaged with CARS. Metabolites were extracted from images samples were analyzed with ToF-SIMS to obtain mass spectrum. Data of four samples and control were compared with multivariate analysis method. siADRP sample showed the most extreme changes in phenotype, and siKIF3A sample changed the most in dynamics compared to control sample.


 활용도 분석

  • 상세보기

    amChart 영역
  • 원문보기

    amChart 영역