Snail의 기능을 조절하는 새로운 kinase의 탐색 및 기능 규명
cancer metastasis Snail;
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The functions of the Snail family zinc-finger transcription factors are essential during embryonic development and tumor progression. One of their best-known functions is to induce epithelial-mesenchymal transition (EMT), which convert epithelial cells into migratory mesenchymal cells. Recently, many orthologues of the Snail family proteins have been identified throughout the animal kingdom and their studies are providing new clues about the EMT-dependent and -independent functions of Snail protein. To find new regulatory mechanism of Snail's activity, Snail interaction proteins were explored by using Mammalian Vector Two-hybrid system. 10 kinase genes were selected as first candidates of Snail-interacting proteins. Results of Mammalian Vector Two-hybrid system were confirmed by co-immunoprecipitation analysis and found that Snail interacts with IKKγ, AKT2, AKT3, GRK5 and ERK3 proteins, respectively. Expression of Snail was positively correlated with that of ERK3 in various cancer cell lines. When the amounts of ERK3 are increased, stability of Snail is increased. And stability of Snail is also increased in the presence of IKKγ compared in the absence of it. If we find proteins that can regulate the function of Snail, these could be good targets for the development of new therapeutic agent that can control of cancer metastasis.