Use and Separation of Volatile Extracts from Forest Biomass of Korea
임산공학과 목재화학 및 펄프, 제지공학 전공
Ⅷ, 60 p.
UES AND SEPARATION OF STEAM EXTRACT FROM BAMBOO LEAVES OF KOREA UES AND SEPARATION OF EXTRACT FROM Coptis chinensis OF KOREA;
- 원문 URL
Natural plant extract have been the subject of intense research aiming at elucidating the underlying mechanisms of their chemopreventive effects on various forms of human cancers. The objective of our study was to evaluate the natural antioxidant and anticancer agent potential of Phyllostachys. Analysis of the chemical composition of the steam extract from Phyllostachys was carried out using GC–MS. The steam extract of Phyllostachys was dominated by monoterpenes (63.0‑71.4%) and sesquiterpenes (23.6‑33.1%) as the main compounds. The antioxidant activities of the steam extract were determined using a DPPH scavenging and hydrogen peroxide scavenging activity test system. Furthermore, the amount of total phenolics in the steam extract was determined spectrometrically. The steam extract of P. pubescens and P. bambusoides showed the highest antioxidant activity (69.4 and,_ 64.0%, respectively). The steam extract from Phyllostachys species showed a hydrogen peroxide scavenging activity of approximately 50.4‑54.6% when compared with that of the standard gallic acid. The anticancer activities of the steam extract were determined using a MTT assay. Assessment of the cytotoxic effect of the steam extract on PC-3 cells showed that the P. bambusoides (20.9%) and P. pubescens (20.4%) were superior in inducing cytotoxicity compared with the steam extract of P. nigra var. henonis (1.2%). Findings from this study indicate that the steam extract of P. bambusoides and P. pubescens possess the potential of a medicinal drug, especially in prostate cancer treatment.Botanical insecticides based on natural compounds from plants are expected to be potential alternatives to traditional chemical pesticides. This study investigated the potential of an extract from Coptis chinensis as an environmentally safe measure to control the peach-potato aphid, Myzus persicae. The fractionation of the extract from Coptis chinensis was sequentially divided into n-hexane, chloroform, and ethyl acetate fractions for the analysis of the chemical composition and use in a bioassay. The insecticidal activity against Myzus persicae was assessed for the crude extract and its various extracts. The crude extract, hexane, chloroform, and ethyl acetate fraction differed significantly among extracts and in concentration, ranging from 12.6‑36.5%, 3.7‑22.2%, 21.7‑39.23% and 7.1‑20.3%, respectively. Among the different fractions, the chloroform fraction was associated with the highest insecticidal activity. The chemical composition of the chloroform fraction was highest in aniline and 2-methoxy-4-vinylphenol. Aniline and 2-methoxy-4-vinylphenol (commercial chemicals) ware used as a positive control for the tested insecticides and differed significantly in there components and concentration, ranging 85.4‑98.1%, and 79.9‑95.4%, respectively. The chloroform fraction of Coptis chinensis showed insecticidal activity, providing evidence for its use as a bio insecticide. This may be a useful starting point for the development of bio insecticides.