오배자와 연교 합제 추출물의 Streptococcus pyogenes 감염 마우스에 대한 항균 및 치료효과
오배자 연교 Streptococcus pyogenes;
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Streptococcus pyogenes (S. pyogenes) is an aerobic, spherical and gram-positive extracellular bacterium that is the cause of group A streptococcal infections and many important human diseases, ranging from mild superficial skin infections to life-threatening systemic diseases. The most important transmission modes of S. pyogenes are via respiratory droplets, hand contact with nasal discharge and skin contact with impetigo lesions. The pathogen of S. pyogenes can be found in its carrier state in the anus, vagina, skin and pharynx and contact with these surfaces can spread the infection. The bacterium can be spread to cattle and then back to humans through raw milk as well as through contaminated food sources such as salads, milk and eggs. However, cattle do not contract the disease. Necrotizing fasciitis is usually because of contamination of skin lesions or wounds with the infectious agent. The present study was investigated the antibacterial effect of Rhus chinensis and Forsythia suspensa extracts against S. pyogenes, and therapeutic effect of a mixture of Rhus chinensis and Forsythia suspensa (1:1) extracts (HCE) for S. pyogenes infection in mice. In the antibacterial effect against S. pyogenes, the inhibition zone diameter of Rhus chinensis and Forsythia suspensa extract was 12 and 13 mm at the concentration of 6.0 mg/ml, respectively. On 8 hours after incubation of S. pyogenes in Brain-Heart Infusion (BHI) broth, HCE at the concentration of 0.5, 1.0 and 2.0 mg/ml was inhibited the growth of S. pyogenes by 15.1, 51.1, and 85.6%, respectively. For 12 days after single challenge with S. pyogenes, forty female BALB/c mice were divided into four experimental groups which were orally administered with saline, 2.5, 5.0, and 10.0 mg/kg HCE, respectively. On the 12th day, the survival rate of mice treated with 2.5, 5.0, and 10.0 mg/kg HCE was 10, 30, and 50%, respectively. The results of this study indicate that HCE has a potential treatment for S. pyogenes infection in mice. To evaluate the safety of the exposure to various doses of HCE in 5-week-old BALB/c mice, forty mice were divided into four equal groups and orally treated with 5.0, 10.0, and 20.0 mg/kg of the combination for 7 consecutive days, respectively. In the hematological and blood biochemical analysis, all parameters in the treated groups were not significantly difference compared to those of the control group. Thus, 7-day administration of 20.0 mg/kg HCE did not affect the hematological and blood biochemical parameters. In conclusion, this study suggested that it was safe to administrate 20.0 mg/kg of HCE, and it is also expected that HCE can be used for the substitution of antibiotics against S. pyogenes infection in mice.