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차세대 염기서열법을 적용한 16S rRNA 유전자의 피로시퀀싱에 의한 화농성 간농양 원인균의 프로파일링 원문보기

  • 저자

    송윤규

  • 학위수여기관

    경상대학교 대학원

  • 학위구분

    국내박사

  • 학과

    의학과 미생물

  • 지도교수

  • 발행년도

    2014

  • 총페이지

    v, 45 p.

  • 키워드

    화농성 간농양 차세대 염기서열법;

  • 언어

    kor

  • 원문 URL

    http://www.riss.kr/link?id=T13534400&outLink=K  

  • 초록

    Pyogenic liver abscess (PLA) is a potentially fatal disease. Understanding the pathogens that cause PLA is the basis for PLA treatment. Here, we profiled the bacterial composition in PLA fluid by pyrosequencing the 16S ribosomal RNA (rRNA) gene based on next-generation sequencing (NGS) technology to identify etiological agents of PLA and to provide information of their 16S rRNA sequences for application to DNA-based techniques in the hospital. Twenty patients with PLA who underwent percutaneous catheter drainage, abscess culture, and blood culture for isolates were included. Genomic DNAs from abscess fluids were subjected to polymerase chain reaction and pyrosequencing of the 16S rRNA gene with a 454 GS Junior System. The abscess and blood cultures were positive in nine (45%) and four (20%) patients, respectively. Pyrosequencing of 16S rRNA gene showed that 90% of the PLA fluid samples contained single or multiple genera of known bacteria such as Klebsiella, Fusobacterium, Streptococcus, Bacteroides, Prevotella, Peptostreptococcus, Dialister, and unassigned Enterobacteriaceaer. Klebsiella was predominantly found in the PLA fluid samples. All samples that carried unassigned bacteria had 26.8% reads on average. We demonstrated that the occurrence of PLA was associated with eight known bacterial genera as well as unassigned bacteria. We provide a bacterial profile for PLA and information on the 16S rRNA sequence to apply molecular-based techniques in the hospital. Additionally, 16S rRNA gene sequencing was more useful than conventional culture methods for accurate identification of bacterial pathogens from PLA.


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