Genome-wide identification and analysis of Catharanthus roseus RLK1-like kinase family in rice
KyungHee University, Graduate School
Dr. Ki-Hong Jung
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Family of Catharanthus roseus receptor like kinase1-like kinases (CrRLK1Ls) is a novel type of RLK, was recently identified in Arabidopsis with 17 members carrying a putative extracellular carbonhydrate-binding malectin-like domain. Function of CrRLK1L family in rice, a most widely consumed staple food, is mostly not discovered. To assign the function of CrRLK1Ls family in rice, we produced the phylogenomic data with the integration of microarray-based anatomical and diurnal expression profiling data using a large number of public microarray data to the context of rice CrRLK1Ls family phylogenic tree. Chromosomal localization of CrRLK1Ls reveals that three of 16 genes were tandem duplicated, suggesting possible functional redundancy within this gene family. However, the integrated diurnal expression suggests the functional divergence of two of three genes: OsCrRLK1L2 showed the peak of expression in the day but OsCrRLK1L3 showed the peak in the night. Interestingly, expression of OsCrRLK1L2 was repressed in osgigantea (osgi) mutant, indicating that OsCrRLK1L2 functions downstream of OsGI. Network analysis associated with OsCrRLK1L2 and OsGI suggests the novel mechanism of circadian regulation mediated by OsGI. In addition, the identification that three out of five root preferred OsCrRLK1L genes were stimulated by drought suggests the roles of this family for water use efficiency. This study provides a global view of CrRLK1Ls in rice to facilitate the functional identification and further applications.