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Study on the effect of blue-light receptor phototropin 1 in the root of Arabidopsis plants. 원문보기

  • 저자

    Moni Akhi

  • 학위수여기관

    울산대학교

  • 학위구분

    국내박사

  • 학과

    생명과학전공

  • 지도교수

    한인섭

  • 발행년도

    2014

  • 총페이지

    66 p.

  • 키워드

    phototropin 1 arabidopsis lateral root;

  • 언어

    eng

  • 원문 URL

    http://www.riss.kr/link?id=T13540286&outLink=K  

  • 초록

    CHAPTER I The blue-light receptor phototropin 1 suppresses lateral root growth by controlling cell elongation. We investigated the relationship between the blue light receptor phototropin 1 (phot1) and lateral root growth in Arabidopsis thaliana seedlings. Fluorescence and confocal microscopy images, as well as PHOT1 mRNA expression studies provide evidence that it is highly expressed in the elongation zone of lateral roots where auxin is accumulating. However, treatment with the auxin transport inhibitor N-1-naphthylphthalamic acid significantly reduced PHOT1 expression in this zone. In addition, PHOT1 expression was higher in darkness than in light. The total number of lateral roots was higher in the phot1 mutant than in wild-type Arabidopsis. Cells in the elongation zone of lateral roots of the phot1 mutant were longer than those of wild-type lateral roots. Further, fungal contamination or high dose NAA significantly decreased phot1 expression may be due over production of auxin in LR. Additionally, phot1 showed negative effects in phototropism and gravitropism in Arabidopsis roots. Furthermore, salinity significantly decreased LR number in phot1 mutants compared to untreated mutants and WT plants respectively, which indicates the inhibition LR formation by salt stress is independent of phot1 and salinity significantly decreased gravitropic response in phototropin mutants. These findings suggest that PHOT1 plays a role(s) in elongation of lateral roots through the control of an auxin-related signaling pathway. CHAPTER II Using Arabidopsis seedlings to teach phototropism and gravitropism in a K-12 laboratory classroom. The purpose of this study was to design a teaching method suitable for science high school students easy to learn how plants respond to stimuli or signals from their environment in order to live successfully. During their scientific inquiry procedure, high school students observed easily about the physiological responses, such as phototropism and gravitropism. The knowledge regarding phototropism and gravitropism helped student to understand how plant can grow up by using environmental resources as well as they learned about differential response of different parts of them. This teaching method enhanced students' science-learning motivation and creativity.


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