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아밀레이즈를 이용한 다공성 전분 입자의 생산 및 특성 분석 : Characterization of porous starch granules produced by starch hydrolyzing enzymes 원문보기

  • 저자

    정이슬

  • 학위수여기관

    세종대학교 대학원

  • 학위구분

    국내석사

  • 학과

    식품공학과

  • 지도교수

    유상호

  • 발행년도

    2014

  • 총페이지

  • 키워드

    Porous starch granules Amylolytic enzymes Holding capacity Tailor-made technique Encapsulation;

  • 언어

    eng

  • 원문 URL

    http://www.riss.kr/link?id=T13541128&outLink=K  

  • 초록

    Porous starch granules (PSGs) with tailor-made size of the pore and cavity were prepared by different amylolytic enzyme and elucidated the structural and physical properties. Starch-active biocatalysts, α-amylase, β-amylases and glucoamylase (800 U/g starch each) were added to starch slurry (5%, w/v) and incubated at 30 or 40°C up to 12 h. The α-amylases-treated PSGs showed the largest pore size (>1200 nm in diameter) and diameter ratio of cavity to granule. Gelatinization temperatures and enthalpies increased in all enzyme-treated PSGs compared to native corn starch (NCS). The onset temperature of α-amylase-, β-amylase- and glucoamylase-treated PSGs were 72.1, 68.7, and 68.1°C after 8 h reaction, respectively. The ΔH was 8.5, 13.4, and 13.1 J/g PSGs as same order. The relative crystallinity of α-amylase-treated PSGs was 26.8%, similar to 27.8% of NCS, and those of β-amylase- and glucoamylase-treated PSGs were 31.1 and 32.1%, respectively. Water holding capacities of α-amylase-, β-amylase-, and glucoamylase-treated PSGs were 209.7, 94.6, and 133.8%, respectively, which were higher than 89.1 of NCS. Oil holding capacities of α-amylase, β-amylase-, and glucoamylase-treated PSGs were 304.5, 182.7, and 211.5%, respectively, which were higher than 161.8% of NCS. Crocin holding yields of α-amylase-, β-amylase-, and glucoamylase-treated PSGs were 25.7, 20.8, and 20.6 mg/g PSGs, respectively, which were higher than 18.6 mg/g of NCS. Curcumin holding yields were 11.1, 5.7, and 7.75 mg/g in same order, which were higher than 4.4 mg/g of NCS. The digestibility of α-amylase-, β-amylase-, and glucoamylase-treated PSGs by simulated human digestive fluids were 69.1, 76.4, and 77.6%, respectively, which were lower than 89.1% of NCS. Also all PSGs were not hydrolyzed at artificial mouth and stomach conditions. It means that the enzyme- treated PSGs can be an effective carrier for transferring functional materials into small intestine. From these results, enzyme types and their respective reaction conditions can be utilized selectively and controlled appropriately to generate desirable sizes of cavities and pores in the starch granules. This enzyme-selective approaches could contribute to develop tailor-made PSGs with distinct internal structure for a specific use in a wide range of food and other industrial applications.


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