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Molecular Cloning and M13 Subcloning of Genes Encoding Catechol Dioxygenases

Kim, Young-Soo    (Department of Biochemistry, College of Pharmacy, Chungbuk National University   ); Choi, Bong-Soo    (Department of Biochemistry, College of Pharmacy, Chungbuk National University   ); Min, Kyung-Rak    (Department of Biochemistry, College of Pharmacy, Chungbuk National University  );
  • 초록

    Achromobacter xylosoxidans KF701 and Pseudomonas putida (NAH7) were significantly different in degradative capability of aromatic compounds including benzoates, biphenyls, and naphthalene. However, both of the bacterial strains can grown on catechol as the sole carbon and energy source. Catechol 2, 3-dioxygenase gene for naphthalene oxidation or biphenyl oxidation was cloned into Escherichia coli HB 701. A E. coli HB 101 clone containing catechol 2, 3-dioxygenase gene from P. putida (NAH7) contains a recombinant plasmid with 3.60kb pBR322 and 6-kb insert DNA. Another E. coli HB101 clone containing catechol 2, 3-dioxygenase gene from A. xylosoxidans KF 701 has a recombinant plasmid with 4.4kb pBR322 and 10-kb insert DNA. Physical maps of the recombinant plasmids were constructed, and catechol 2, 3-dioxygenase gene in the recombinant plasmide was further localized and subcloned int M13. The cloned-catechol 2, 3-dioxygenase game products were identified as yellow bands on nondenaturaing polyacrylamide gel after electrophoresis followed by activity staining with catechol solution.


  • 주제어

    Aromatic pollutant .   naphthalene .   biphenyl catechol dioxygenase .   gene cloning.  

 저자의 다른 논문

  • Kim , Young-Soo (67)

    1. 1989 "5-Lipoxygenase의 활성과 Leukotriene $B_4$ 생합성 억제물질" 약학회지 = Yakhak hoeji 33 (6): 319~323    
    2. 1990 "흰쥐의 다리종양에서 발현된 케이라스 암유전자" 한국생화학회지 23 (2): 185~189    
    3. 1991 "Pseudomonas putida의 Catechol 2,3-dioxygenase 유전자의 클로닝" Korean journal of microbiology = 미생물학회지 29 (3): 155~159    
    4. 1992 "Cloning and Overexpression of Methylcatechol 2, 3-Dioxygenase Gene from Toluene-Degrading Pseudomonas putida mt-2(pWWO)" Archives of pharmacal research : a publication of the Pharmaceutical Society of Korea 15 (4): 360~364    
    5. 1995 "Inhibitory Effects of Herbal Medicines on Hyaluronidase Activity" 생약학회지 26 (3): 265~272    
    6. 1995 "Effect of Temperature on Persistence of Recombinant Plasmid pCU103 in Different Waters" The journal of microbiology 33 (3): 178~183    
    7. 1996 "Inhibitory Effects of Triterpenoids on Interleukin-8/CINC-1 Induction in LPS-Stimulated Rat Peritoneal Macrophages" Natural product sciences 2 (1): 48~55    
    8. 1996 "Inhibitory Effects of Herbal Extracts on Cyclooxygenase Activity of Prostaglandin $H_2$ Synthase from Sheep Seminal Vesicle" Natural product sciences 2 (1): 56~74    
    9. 1996 "Extracellular DNAs Released form the Genetically Engineered E. coli CU103 During Growth in Different Liquid Media" The journal of microbiology 34 (2): 144~150    
    10. 1996 "Cloning and Expression of Pseudomonas cepacia catB Gene in Pseudomonas putida" The journal of microbiology 34 (4): 334~340    
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