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A Turbidimetric Determination of Protein by Trichloroacetic Acid

Choi, Wahn-Soo   (Central Researchand Development Laboratory, Yung Jin Pharmaceutical Co, Ltd.,  ); Chung, Kae-Jong   (Central Researchand Development Laboratory, Yung Jin Pharmaceutical Co, Ltd.,  ); Chang, Man-Sik   (Central Researchand Development Laboratory, Yung Jin Pharmaceutical Co, Ltd.,  ); Chun, Jae-Kwang   (Central Researchand Development Laboratory, Yung Jin Pharmaceutical Co, Ltd.,  ); Lee, Hyang-Woo   (Department of Pharmacy, Sungkyunkwan UniversityUU0000759  ); Hong, Sung-Youl   (Department of Genetic Engineering, Sungkyunkwan UniversityUU0000759  );
  • 초록

    Based on the turbidimetric response of protein with 50% trichloroacetic acid (TCA), this study aims to introduce an assay method for protein in solution. The standard procedure consists of mixing equal volume of sample solution (standard or unknown) with 50%-TCA solution and measuring the absorbance at 450 nm after 20 min. The absorbances of the solutions were almost stable over 120 min at room temperature. This assy method is simple, reproducible, and tolerant to many interfering substances. It can detect less amount than $10\mu$ g/ml of bovin serum albumin. The assay method has low protein-to-protein variability over wide range of molecular weight.


  • 주제어

    Turbidimetry .   Trichloroacetic acid .   Protein determination.  

 저자의 다른 논문

  • Lee, Hyang-Woo (50)

    1. 1985 "Studies on unknown methylated compounds of non-histone nuclear protein" Archives of pharmacal research : a publication of the Pharmaceutical Society of Korea 8 (3): 149~157    
    2. 1985 "Protein methylase II에 의한 myelin 염기성 단백질의 carboxyl-methylesterification" 한국생화학회지 18 (2): 152~156    
    3. 1986 "HPLC Analysis of Methylated Amino Acids : Methylated Amino Acids on HPLC" Archives of pharmacal research : a publication of the Pharmaceutical Society of Korea 9 (1): 15~18    
    4. 1986 "Fractionation of enzymatically methylated acid-insoluble proteins from thymus nuclei" Archives of pharmacal research : a publication of the Pharmaceutical Society of Korea 9 (3): 157~161    
    5. 1987 "Endogenous Proteinaceous Inhibitor for Protein Methylation Reactions" Archives of pharmacal research : a publication of the Pharmaceutical Society of Korea 10 (3): 193~196    
    6. 1987 "돼지 췌장내 Protein Methylase I의 분리정제 및 성질" 약학회지 = Yakhak hoeji 31 (3): 173~181    
    7. 1987 "신경세포의 Myelination에 있어서 Myelin Basic Protein의 Methyl화 현상에 관한 연구" 약학회지 = Yakhak hoeji 31 (5): 266~272    
    8. 1988 "Cytochrome C methylation: Current Knowledge of its Biological Significance" Archives of pharmacal research : a publication of the Pharmaceutical Society of Korea 11 (1): 7~13    
    9. 1988 "Euglena의 Cytochrome C552 Methylation에 관한 연구" 약학회지 = Yakhak hoeji 32 (6): 420~427    
    10. 1989 "Effect of Enzymatic Methylation of Proteins on Their Isoelectric Points" Archives of pharmacal research : a publication of the Pharmaceutical Society of Korea 12 (2): 79~87    
  • Hong, Sung-Youl (46)

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