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Effects of Radioprotectors on DNA Repair Capacity of Tumor Cells

Kim, Choon-Mi   (College of Pharmacy, Ewha Womans UniversityUU0001056  ); Kim, Mi-Kyung   (College of Pharmacy, Ewha Womans UniversityUU0001056  );
  • 초록

    Three cell lines, CHO, L929 and B16 which are non-tumorigenic and cancer cells, respectively, were first tested for their survival in the presence of radioprotective ginseng protein fraction(GPF0. The influence of three radioprotectors-CPF, cysteamine, and 1-Methyl-2-bis[(2-methylthio)vinyl] quinolinium iodide (MVQI) on DNA repair capacity of UV damaged cells survival test, the GPF showed higher cytotoxicity in L929 and B16 than in CHO cells. However, the degree of cell killing was also investigated by measuring $^3H$ -thymidine incorporation of PUVA treated cells. In cell survival test, the GPF showed higher cytotoxicity in L929 and B16 than in CHO cells. However, the degree of cell killing was not high enough to consider it as an antitumorigenic agent. Variable results were obtained in the effects on DNA repair capacity depending on the protectors and cell lines used. In pretreatment, the presence of GPF and MVOI brought about a sinificant increase in the capacity in both CHO and B16 cells. However, in L929, the enhancing effect was not shown. In all three cell lines, cysteamine showed lower repair capacity than control, suggesting the primary damage reduction in stronger enhancing effects in L929 and B16 cells, while it was weaker in CHO cells. Here also cystemine hsowed a very little or no increase in the capacity in all three cell lines. These results demonstrate that GPF has mild cytotoxicity in tumorignic cells and that GPF and MVQI enhance DNA repair capacity of UV damaged cells, whether they are tumorigenic or not. On the other hand, cysteamine shows only damage reduction effect. Celles of different genetic origin seem to give different responses to the modifier and different modifiers may possibly work by different mechanisms.


  • 주제어

    Radioprotectors .   Cell survival .   UV damage .   DNA repair capacity .   PUVA treatment .   $^3H$-thymidine incorporation.  

 저자의 다른 논문

  • Kim, Choon-Mi (22)

    1. 1985 "인삼단백질의 방사선 방어작용" 약학회지 = Yakhak hoeji 29 (5): 246~252    
    2. 1986 "Study on the molecular weights of radioprotective ginseng proteins by HPLC method" Archives of pharmacal research : a publication of the Pharmaceutical Society of Korea 9 (1): 5~9    
    3. 1986 "인삼 단백질분획에 대한 폴리아크릴아미드 전기영동" 약학회지 = Yakhak hoeji 30 (6): 343~347    
    4. 1988 "Effects of Ginseng Protein on Relative Survival and Chromosome Aberration of UV Irradiated Cells" Archives of pharmacal research : a publication of the Pharmaceutical Society of Korea 11 (3): 225~229    
    5. 1988 "방사선 방어작용이 있는 인삼 단백분획의 CHO-KI 세포에 대한 세포 독성" 약학회지 = Yakhak hoeji 32 (5): 313~318    
    6. 1988 "Effects of Radioprotective Ginseng on Protein UV induced Sister Chromatid Exchanges" Archives of pharmacal research : a publication of the Pharmaceutical Society of Korea 11 (2): 93~98    
    7. 1988 "방사선 방어효과가 있는 인삼단백성분이 MMS에 의해 유도된 자매염색분체 교환과 염색체이상에 미치는 영향" 한국생화학회지 21 (4): 525~530    
    8. 1989 "Gel filtration에 의한 한방사선 인삼단백 분획의 정제" 고려인삼학회지 = Korean journal of ginseng science 13 (2): 254~259    
    9. 1991 "항방사선 인삼분획의 butanol 추출물과 수용성 성분이 세포 생존율에 미치는 영향" 고려인삼학회지 = Korean journal of ginseng science 15 (3): 167~170    
    10. 1992 "인삼 사포닌이 일산화탄소중독 및 노화과정에서 흰쥐의 신경전달물질 함량 변화에 미치는 영향" 약학회지 = Yakhak hoeji 36 (3): 285~290    

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