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Elicitors which Induce the Accumulation of p-Coumaroylamino Acids in Ephedra distachya Cultures

Song, Kyung-Sik    (Genetic Engineering Research Institute, Korea Institute of Science and Technology   ); Sankawa, Ushio    (Faculty of Pharmaceutical Science, The University of Tokyo   ); Ebizuka, Yutaka    (Faculty of Pharmaceutical Science, The University of Tokyo  );
  • 초록

    Some ammonium oxalate soluble pectic fragments prepared from cultured cell wall of Ephycla distrahya elicited the accumulation of p-coumarocylamino acids (p-CAA) in E. distachya cultures while water soluble and alkali soluble fractions had no activity. Partial purification of the pectic fragments fraction using DEAE-cellulose chromatography afforded two active fractions (PS-I and PS-II) which were composed of mainly uronic acids (98-99 w/w %). They elicited the accumulation of p-CAA in an amount of 52-60 nmol per gram fresh weight of cultures. The acidic sugar compositions of PS-I and PS-II were found to be galacturonic acid and glucuronic acid by TLC analysis. They were supposed to act as endogenous elicitors of p-CAA accumulation. In order to investigate the effect of ethylene on p-CAA accumulation, Ethrel, which is known as ethylene generator, and ACC(1-aminocyclopropane-1-carboxylic acid), a direct precusor of ethylene biosynthesis, were added to the culture. However, they did not glycopeptide elicitor [(Con A-II)], either. Consequently, no relationships between ethylene and p-CAA accumulation were recognized. Several tentative elicitors were teted for their activity. Commercial yeast glucan, $CuCl_2$ , laminarin and laminariheptaose had slight activity whereas ${\alpha}$ -methylmannopyranoside and commercial yeast mannan had no elicitor activity. ${\alpha}$ -methylmannopyranoside which has been known as a tentative inhibitor of glucan elicitor in Glycine max did not affect on the elicitor activity of Con A-II.


  • 주제어

    Ephedra distachya cultures .   Endogenous elicitor .   Cell wall pectic substances .   Ethylene .   p-coumaroylamino acids.  

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