본문 바로가기
HOME> 논문 > 논문 검색상세

논문 상세정보

2,4,5-Trichlorophenoxyacetic Acid 분해균주의 분리 및 특성
Isolation and Characterization of 2,4,5-Trichlorophenoxyacetic Acid Degrading Bacteria

박영순    (부산대학교 미생물학과   ); 이건    (부산대학교 미생물학과   ); 이상준    (부산대학교 미생물학과   ); 이종근    (부산대학교 미생물학과  );
  • 초록

    Microorganisms capable of utilizing 2,4,5-trichlorophenoxyacetic acid(2,4,5-T) as sole carbon source were isolated from soil by enrichment culture. Among these strains, EL-O7IP had the highest biodegradability of 2,4,5-7, and according to its morphological and physiological characteristics, it was identified as Pseudomonas sp. This strain was resistant to rifampicin, streptomycin, ampicillin, kanamycin and such metal ions as $Zn^{2+}$ , $Cu^{2+}$ Various compounds of chlorinated phenol and substrate analogs were more easily utilized than 2,4,5-7, but biodegradation rate for each compound was different. The strain easily utilized the compounds of chlorinated substituents on phenol in the order of ortho-, para-, and meta- position. The biodegradability of this strain was very stable. Key words : 2,4,5- trichlorophenoxyacetic acid, Pseudomonas sp .


  • 주제어

    2,4,5-trichlorophenoxyacetic acid .   Pseudomonas sp.  

  • 참고문헌 (30)

    1. Metabolism of halophenols by 2,4,5-T degrading Pseudomonas cepacia , Karns,J.S.;J.J.Kilbane;S.Duttagupta;A.M.Chakrabarty , Appl. Environ. Microbiol. / v.46,pp.1176-1181,
    2. Manupulation of degradative genes of soil bacteria , Franklin,F.C.H.;M.Bagdasrain;K.N.Timmis;T.Leisinger(ed.);R.Hutter(ed.);A.M.Cook(ed.);J.Nuesch(ed.) , Microbial degradation of xenobiotics and recalcitrant compounds / v.,pp.109-130,
    3. Microbial metabolism of 2,4,5-T in soil, soil suspensions and axenic culture , Rosenberg,A.;M.Alexander , J. Agric. Food. Chem. / v.28,pp.297-302,
    4. Dechlorination of 2,4,5-T by an anaerobic microorganism , Suflita,J.M.;J.Stout;J.M.Tiedje , J. Agric. Food Chem. / v.32,pp.218-221,
    5. Aerial spraying of 2,4,5-T and human birth malformation : on epidemiological investigation , Hanify,J.A.;P.Metcalt;C.L.Nobbs;K.J.Worsley , Science / v.212,pp.349-351,
    6. Gerhardt,P.;R.G.E.Murray;R.N.Costilow;E.W.Nester;W.A.Wood;N.R.Krieg;G.B.Phillips , Manual of Methods for General Bacteriology / v.,pp.,
    7. The William and Wilkins Co. , Bergey's Manual of Determinative Bacteriology(8th ed.) / v.,pp.,
    8. Degradation of herbicide mecoprop (2-(2-methyl 4-chlorophenoxy) propionic acid) by a synergistic microbial community , Hilary,M.C.;P.G.Michael;J.H.Slater , Appl. Environ. Microbiol. / v.49,pp.429-433,
    9. 2,4,5-T Decomposition on tropical soil and its cometabolism by bacteria in vitro , Rosenberg,A.;M.Alexander , J. Agric. Food Chem. / v.28,pp.705-709,
    10. The William and Wilkins Co. , Bergey's Manual of Determinative Bacteriology / v.1,pp.,
    11. Bradshow,L.J. , Laboratory Microbiology(3rd ed.) / v.,pp.,
    12. Isolation of large bacterial plasmids and characterization of the P2 incompatibility group plasmids pMG1 and pMG5 , Hansen,J.B.;R.H.Olsen , J. Bacteriol. / v.135,pp.227-238,
    13. Pesticides, polych-lorinated-biphenyls, and other chloroorganic compounds , Bailey,R.A.;H.M.Clarke;J.P.Ferris;S.Krause;R.L.Strong , Chemistry of the environment / v.,pp.147-193,
    14. Biotransformation of pesticides and other xenobiotics in plants and soils-recent developments , Edwards,V.T.;A.C.McMinn;D.H.Hutson(ed.);T.R.Roberts(ed.) , Progress in pesticide biochemistry and toxicology / v.4,pp.,
    15. Plasmid in the biodegradation of chlorinated aromatic compounds , Chatterjee,D.K.;S.T.Kellogg;D.R.Watkins;A.M.Chakrabarty;S.B.Levy(ed.);R.C.Clowes(ed.);E.L.Koening(ed.) , Molecular biology, pathogenicity, and ecology of bacterial plasmids / v.,pp.519-527,
    16. Compunds interfering with cell growth, division, and development , Corbelt,J.R.;K.Wright;A.C.Baillie , The biochemical mode of action of pesticides(2nd ed.) / v.,pp.179-195,
    17. Biodegradation of 2,4,5-T in soil by a pure culture of Pseudomonas cepacia , Chatterjee,D.K.;J.J.Kilbane;A.M.Chakrabarty , Appl. Environ. Microbiol. / v.44,pp.514-516,
    18. Fate of uniformly carbon-14-ring labelled 2, 4, 5-T and 2, 4-D , McCall,P.J.;S.A.Vrona;S.S.Kelley , J. Agric. Food Chem. / v.29,pp.100-107,
    19. The Genotoxic Effects of 2,4,5-T , Grant,W.F. , Mutat. Res. / v.65,pp.83-119,
    20. Detoxification 2,4,5-T from contaminated soil by Pseudomonas cepacia , Kilbane,J.J.;D.K.Chatterjee;A.M.Chakrabarty , Appl. Environ. Microbiol. / v.45,pp.1697-1700,
    21. Biodegradation of chemicals of environmental concern , Alexander,M. , Science / v.211,pp.132-138,
    22. Microbial degradation of halogenated compounds , Chakrabarty,A.M.;D.Ghosal;I.S.You;D.K.Chatterjee , Science / v.288,pp.135-142,
    23. Isolation and characterization of bacteria degrading chlorinated aromatic hydrocarbon , Kim,J.W.;C.K.Kim;T.H.Yeom;J.G.Lee , Kor. Jour. Microbial. / v.25,pp.122-128,
    24. MacFaddin,J.F. , Biochemical test for identification of medical bacteria / v.,pp.,
    25. Regulation of 2,4,5-T and chlorophenol metabolism in Pseudomonas cepacia AC 1100 , Karns,J.S.;S.Duttagupta;A.M.Chakrabarty , Appl. Environ. Microbiol. / v.46,pp.1182-1186,
    26. Rapid screen for bacteria degrading water-insoluble, solid hydrocarbons on agar plate , Kiyohara,H.;K.Nagao;K.Yana , Appl. Environ. Microbiol. / v.41,pp.454-457,
    27. 장판섭 , 최신 신농약 / v.,pp.286-295,
    28. Biodegradation of 2,4,5-T by a pure culture of Pseudomonas cepacia , Kilbane,J.J.;D.K.Chatterjee;J.S.Karns;S.T.Kellogg;A.M.Chakrabarty , Appl. Environ. Microbiol. / v.44,pp.72-78,
    29. Plasmid-assisted molecular breeding: new technique for enhanced biodegradation of persistent toxic chemicals , Kellogg,S.T.;R.K.Chatterjee;A.M.Chakrabarty , Science / v.214,pp.1133-1135,
    30. Microbial cometabolism of 2,4,5-T , Horvath,R.S. , Bull. Enviro. Contam. Toxicol. / v.5,pp.537-541,

 저자의 다른 논문

  • 이건 (13)

    1. 1991 "토양에서 분리한 Phenol 분해세균의 수치분류" 산업미생물학회지 = Korean journal of applied microbiology and biotechnology 19 (6): 624~630    
    2. 1992 "2, 4, 5-Trichlorophenoxyacetic Acid 분해균의 유전적 특성에 관한 연구" Korean journal of microbiology = 미생물학회지 30 (4): 260~264    
    3. 1992 "난분해성 할로겐 치환 Phenol의 분해" 한국환경위생학회지 = Korean journal of Environmental Health Society 18 (2): 92~101    
    4. 1993 "Pseudomonas sp. EL-091S에 의한 4-Chlorophenol의 분해 Kinetics" 한국환경과학회지 = Journal of the environmental sciences 2 (2): 95~102    
    5. 1996 "Emulsification of Crude Oil by Acinetobacter sp. SH-14" The journal of microbiology 34 (4): 363~369    
    6. 1997 "Micrococcus sp. M1에 의한 Phenol과 p-Creso의 생분해 Kinetics" 한국환경과학회지 = Journal of the environmental sciences 6 (2): 153~163    
    7. 1997 "2,4,4l-Trichloro-2l-Hydroxydiphenyl Ether 분해균의 분리 및 분해특성" 한국환경과학회지 = Journal of the environmental sciences 6 (2): 173~182    
    8. 1998 "Actinobacillus sp. EL-9로부터 생산된 생분해성 폴리에스터의 구조 및 특성" 생명과학회지 = Journal of life science 8 (5): 526~531    
    9. 1999 "Purification and Characterization of Biosurfactants Produced by Pseudomonas sp. SW1" Journal of microbiology and biotechnology 9 (1): 56~61    
    10. 1999 "Pseudomonas sp. Sw1이 생산하는 Biosurfactant의 조성 및 특성" 산업미생물학회지 = Korean journal of applied microbiology and biotechnology 27 (1): 41~45    
  • Lee, Sang-Joon (82)

  • 이종근 (11)

 활용도 분석

  • 상세보기

    amChart 영역
  • 원문보기

    amChart 영역

원문보기

무료다운로드
  • NDSL :
유료다운로드

유료 다운로드의 경우 해당 사이트의 정책에 따라 신규 회원가입, 로그인, 유료 구매 등이 필요할 수 있습니다. 해당 사이트에서 발생하는 귀하의 모든 정보활동은 NDSL의 서비스 정책과 무관합니다.

원문복사신청을 하시면, 일부 해외 인쇄학술지의 경우 외국학술지지원센터(FRIC)에서
무료 원문복사 서비스를 제공합니다.

NDSL에서는 해당 원문을 복사서비스하고 있습니다. 위의 원문복사신청 또는 장바구니 담기를 통하여 원문복사서비스 이용이 가능합니다.

이 논문과 함께 출판된 논문 + 더보기