본문 바로가기
HOME> 논문 > 논문 검색상세

논문 상세정보

Cyclophosphamide의 면역독성 검출을 위한 in vitro 시험법의 개발
A Development of Methods for detecting Immunosuppression induced by Cyclophosphamide in vitro

정태천    (한국화학연구소 안전성연구부   ); 차신우    (한국화학연구소 안전성연구부   ); 하창수    (한국화학연구소 안전성연구부   ); 한상섭    (한국화학연구소 안전성연구부   ); 노정구    (한국화학연구소 안전성연구부  );
  • 초록

    A splenocyte culture system supplemented with liver microsomes was developed to detect immunotoxic chemicals which require metabolic activation using cyclophosphamide as a positive standard. When liver microsomes were added to splenocyte cultures isolated from female B6C3Fl mice, the proliferation of splenocytes by lipopolysaccharide (LPS) was increased and the proliferation by concanavalin A (Con A) was decreased. However, when compared with each corresponding control, cyclophophamide was successfully activated to metabolites capable of suppressing Iymphoproliferative responses. This suppression was clearly dependent upon the amounts of microsomes added and/or the concentration of cyclophosphamide exposed. In these cultures, the proliferation of splenocytes was suppressed when the cells were exposed to cyclophosphamide on the day of culture initiation. On the other hand, microsome was responsible for the increase in LPS mitogenicity and NADPH was responsible for the decrease in Con A mitogenicity. Finally, our present culture system was compared with the hepatocyte-splenocyte coculture system which we had developed earlier. We found that the hepatocyte-splenocyte coculture was better able to activate cyclophosphamide to metabolites capable of suppressing the antibody response to sheep erythrocytes. Although our present culture system was relatively poor to activate cyclophosphamide in cultures for antibody response, it will be useful as a simple screening method to detect suppression of certain in vitro immunotoxic parameters like LPS mitogenicity by chemicals which require metabolism.


  • 주제어

    cyclophosphamide .   metabolic activation .   immunosuppression .   splenocyte cultures .   liver microsomes.  

 저자의 다른 논문

  • 차신우 (21)

    1. 1993 "The Effects of KR-10876, a new Quinolone Antimicrobial Agent, on the Central Nervous System" Archives of pharmacal research : a publication of the Pharmaceutical Society of Korea 16 (1): 6~12    
    2. 1993 "Acute Toxicity Study of Recombinant Human Interferon ${\alpha}A$ (LBD-007) in ICR Mice" The journal of applied pharmacology : the official journal of the Korean Society of Applied Pharmacology 1 (2): 266~269    
    3. 1993 "Acute Toxicity Study of Recombinant Granulocyte-Macrophage Colony Stimulating Factor (LBD-005) in ICR mice" The journal of applied pharmacology : the official journal of the Korean Society of Applied Pharmacology 1 (2): 270~274    
    4. 1993 "시험관내에서 captafol이 혈액학, 적혈구막 및 혈장생화학치에 미치는 영향" 大韓獸醫學會誌 = Korean journal of veterinary research 33 (3): 507~511    
    5. 1995 "Beagle dog에 있어서 rifapentine의 독성동태연구" 大韓獸醫學會誌 = Korean journal of veterinary research 35 (4): 815~822    
    6. 1996 "A Study on Antigenicity of Recombinant Human Interferon $\beta$ (LB00013) in Mice and Guinea Pigs" 한국독성학회지 = The Korean journal of toxicology 12 (1): 81~86    
    7. 1996 "영지의 단백다당체 G009의 마우스와 기니픽에 있어서의 항원성에 관한 연구" The journal of applied pharmacology : the official journal of the Korean Society of Applied Pharmacology 4 (1): 1~6    
    8. 1996 "Anthracycline계 항암성 항생물질 DA-125의 Beagle dog에 대한 26주 반복정맥투여독성시험" The journal of applied pharmacology : the official journal of the Korean Society of Applied Pharmacology 4 (2): 127~137    
    9. 1996 "항암제 methotrexate가 랫드 담즙중 환원형엽산유도체의 배설동태에 미치는 영향" 大韓獸醫學會誌 = Korean journal of veterinary research 36 (1): 57~63    
    10. 1997 "비글견에 있어서 새로운 안트라싸이클린계 항암제 DA-125의 정소독성연구" 大韓獸醫學會誌 = Korean journal of veterinary research 37 (2): 425~438    
  • 하창수 (24)

  • 한상섭 (50)

  • 노정구 (41)

 활용도 분석

  • 상세보기

    amChart 영역
  • 원문보기

    amChart 영역

원문보기

무료다운로드
  • NDSL :
유료다운로드

유료 다운로드의 경우 해당 사이트의 정책에 따라 신규 회원가입, 로그인, 유료 구매 등이 필요할 수 있습니다. 해당 사이트에서 발생하는 귀하의 모든 정보활동은 NDSL의 서비스 정책과 무관합니다.

원문복사신청을 하시면, 일부 해외 인쇄학술지의 경우 외국학술지지원센터(FRIC)에서
무료 원문복사 서비스를 제공합니다.

NDSL에서는 해당 원문을 복사서비스하고 있습니다. 위의 원문복사신청 또는 장바구니 담기를 통하여 원문복사서비스 이용이 가능합니다.

이 논문과 함께 출판된 논문 + 더보기