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돼지에서 Actinobacillus pleuropneumoniae의 혈청학적 진단법에 대한 비교연구
Comparative Studies on Serological Tests for Actinobacillus pleuropneumoniae Infection in Swine

심항섭    (경기도 가축위생시험소 북부지소   ); 우종태    (경기도 가축위생시험소 북부지소   ); 조중현    (경기도 가축위생시험소 북부지소   ); 전무형    (충남대학교 수의과대학  );
  • 초록

    To establish an effective diagnostic measure for detection of the antibodies against Actinobacillus pleuropneumoniae, the methods for tube agglutination test (TAT), plate agglutination test (PAT), micro-agglutination test(MAT) and agar-gel immunodiffusion test(ID) were improved and standarized, and the comparative studies were carried out. The results obtained through the experiments were summarized as follows. 1. The rabbit hyperimmune sera to reference serotypes 1 to 6 were cross-tested with TAT, PAT, MAT and ID. In the homologous systems, the range of antibody titers in TAT was 80 to 640, showing the cross-reaction in serotypes 3, 4, 5 and 6. The range of antibody titers in PAT was 4 to 64, showing the cross-reaction in serotypes 3, 4, 5 and 6. In ID, the range of antigen titers was 8 to 32, and cross-reaction was observed in serotype 5. 2. The optimal concentration of antigen in PAT and MAT were 100mg /ml and 1.25mg /ml respectively. The most sensitive reaction in MAT was observed in 52 $^{\circ}C$ for 18hrs. 3. In ID, the most promising antigen and the buffer for agar-gel were EDTA-treated antigen and 0.05M tris buffer (pH 7.2), respectively. 4. By the tests for 200 swine sera, it was found that the frequency of positive reaction were 203 in TAT, 240 in PAT and 163 in ID. 5. When compared the titers of TAT with those of MAT for 200 swine sera, MAT showed the higher titer than TAT being increased by relative correlation. Int was found that the titer for positive readings were 20 in TAT and 40 in MAT. 6. when compared the results of ID with those of TAT for 200 swine sera, all sera with TAT titer under 10 were negative in ID. Of the sera with TAT titer 20 and 40, 55.1% nd 91.8% were positive in ID, respectively. All sera with TAT titer above 80 were positive in ID. In comparison of ID and MAT, all sera with MAT titer under 20 were negative in ID. Of the sera with MAT titer 40 and 80, 24.7% and 93.9% were positive in ID, respectively. All sera with MAT titer over 160 showed positive in ID. 7. In conclusion, the established MAT showed high sensitivity but low specificity, wherease ID revealed low sensitivity but high specificity.


  • 주제어

    Actinobacillus pleuropneumoniae .   tube agglutination test .   micro-agglutination test .   agar-gel immunodiffusion test.  

 저자의 다른 논문

  • 전무형 (85)

    1. 1984 "면양태아피부추출액(緬羊胎兒皮膚抽出液)의 세포면역(細胞免疫) 기능저하(機能低下) 효과(效果)에 관(關)한 연구(硏究)" 農業技術硏究報告 = Research reports of agricultural science and technology 11 (2): 344~351    
    2. 1984 "돼지 알파인터페론 ($\alpha$-interferon)생산 -유전공학기술 응용 기초연구-" 대한수의사회지 = Journal of the Korean Veterinary Medical Association 20 (4): 193~198    
    3. 1984 "도입우에 다발하는 바이러스성 질병" 대한수의사회지 = Journal of the Korean Veterinary Medical Association 20 (6): 321~328    
    4. 1985 "돼지 Leucocyte Interferon 생산(生産)에 대한 기초연구(基礎硏究)" 農業技術硏究報告 = Research reports of agricultural science and technology 12 (1): 153~161    
    5. 1985 "견에 발생한 Leydig Cell Tumor의 증예보고" 대한수의사회지 = Journal of the Korean Veterinary Medical Association 21 (1): 23~27    
    6. 1986 "개의 임파육종의 1예" 韓國臨床獸醫學會誌= Korean journal of veterinary clinical medicine 3 (2): 309~316    
    7. 1986 "면양(緬羊) Squamous Cell Carcinoma의 세포추출액(細胞抽出液)중에 함유된 종양특이(腫瘍特異) 및 면역기능저하물질(免疫機能低下物質)" 農業技術硏究報告 = Research reports of agricultural science and technology 13 (2): 299~310    
    8. 1986 "개와 고양이의 피부사상균 보균실태조사" 대한수의사회지 = Journal of the Korean Veterinary Medical Association 22 (1): 39~45    
    9. 1986 "유전공학기술을 이용한 가축질병의 진료와 예방" 대한수의사회지 = Journal of the Korean Veterinary Medical Association 22 (4): 196~210    
    10. 1987 "가축질병진료와 면역요법의 응용성" 韓國臨床獸醫學會誌= Korean journal of veterinary clinical medicine 4 (1): 439~444    

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