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총담관결찰후 집토끼 Paneth세포의 변화에 대한 면역전자현미경적 연구
Immunoelectron Microscopic Study on the Paneth Cell of Rabbit after the Common Bile Duct Ligation

박경호   (순천향대학교 의과대학 해부학교실UU0000848  ); 조휘동   (순천향대학교 의과대학 해부학교실UU0000848  ); 양남길   (순천향대학교 의과대학 해부학교실UU0000848  ); 안의태   (순천향대학교 의과대학 해부학교실UU0000848  ); 고정식   (순천향대학교 의과대학 해부학교실UU0000848  ); 김진국   (순천향대학교 의과대학 해부학교실UU0000848  );
  • 초록

    Lysozyme has been reported to be present in the secretory granules of the Paneth cell, and lysozyme immunoreactivity has been detected by immunogold method in Paneth cells of the intestine of human, mouse and rat. The present study was aimed at clarifying the intracellular distribution and changes of the lysozyme immunoreactivity in rabbit Paneth cell after common bile duct ligation of rabbit, using the electron microscope immunogold technique. Healthy adult rabbits weighing about 2kg body weight were divided into normal and bile duct ligated groups. Common bile duct ligation was performed aseptically under ether anesthesia. Experimental animals were sacrificed on the 1st, the 3rd, the 5th, the 7th and the 14th day after the operation. Mucosal specimens from the intestinal gland of ileum were fixed in 2.5% glutaraldehyde-1.5% paraformaldehyde, followed by 1% osmium tetroxide, embedded in araldite mixture, cut with LKB-V ultratome. Ultrathin sections were placed on parlodion coated nickel grids (200mesh). The section-bearing grids were floated upside down on the added substance in a moist chamber at room temperature except for the primary antibody step, which was at $4^{\circ}C$ . Sections were etched with a saturated solution of sodium m-periodate for 60min. After etching, sections were pretreated with 0.02M tris buffered saline (TBS), pH 8.4, with 1% bovine serum albumin (BSA, Sigma) for 60min, then treated polyclonal rabbit anti-human lysozyme (Dakipatts) diluted 1 : 50 in TBS with 0.1% BSA for 20hr. Subsequently, grids were incubated 60min in biotinylated goat anti rabbit IgG (Amersham) diluted 1 : 100 in TBS with 0.1% BSA. After this, sections were incubated 60min on streptavidin gold G10 (Amersham) diluted 1 : 50 in TBS with 0.1% BSA. After each step, the grids were briefly rinsed with TBS with 0.1% BSA. After the strepavidin gold step, the sections were jet washed with distilled water. Counterstain of the sections performed by uranyl acetate and lead citrate, and observed with JEM 100 CX II electron microscope. Observed results were as follow; 1. Secretory granules of mouse Paneth cells have a lysozyme immunoreactivity and also eosinophil leucocyte of rabbit applied for the positive-control stain, are well labeld with gold particles. 2. Normal rabbit Paneth cells have a lysozyme immunoreactivity restricted on the secretory granules. 3. Amount lysosomes containing myelin figures in the Paneth cells were significantly increased from 5th day after the common bile duct ligation. 4. Immunoreactivity of Paneth cell secretory granules were more activated on the 3rd day after the common bile duct ligation as compared with those of the normal animal. But the lysozyme immunoreactivity were decreased from the 5th day after the common bile duct ligation. 5. Considering the above finding, lysozyme contained Paneth cell are affected following of common bile duct ligation, whereas lysosomes containing myelin-figure do not exhibit any immunoreactive relationship with those of secretory granules.


  • 주제어

    Bile Duct Ligation .   Paneth Cell .   Immunoelectron Microscope .   Lysozyme .   Immunogold.  

 저자의 다른 논문

  • 박경호 (32)

    1. 1987 "연령에 따른 흰쥐 신사구체의 변화에 대한 전자현미경적 연구" 한국전자현미경학회지 = Korean journal of electron microscopy 17 (1): 47~64    
    2. 1988 "중격측좌핵과 선조체 기저핵의 비교연구 II. 신경 연접형의 구분" 한국전자현미경학회지 = Korean journal of electron microscopy 18 (1): 21~34    
    3. 1988 "중격측좌핵과 선조체 기저핵의 비교연구 III. 유두체 손상과 해마제거의 영향" 한국전자현미경학회지 = Korean journal of electron microscopy 18 (1): 35~48    
    4. 1990 "총담관 결찰후 집토끼 십이지장내 Enterochromaffin Cell의 미세구조적 변화" 한국전자현미경학회지 = Korean journal of electron microscopy 20 (1): 36~50    
    5. 1990 "총담관 결찰이 집토끼 십이지장 점막내 배상세포의 미세구조에 미치는 영향" 한국전자현미경학회지 = Korean journal of electron microscopy 20 (1): 51~64    
    6. 1992 "방사선이 흰쥐 갑상샘 소포곁세포에 미치는 영향에 대한 미세구조적 연구" 한국전자현미경학회지 = Korean journal of electron microscopy 22 (2): 1~14    
    7. 1992 "총담관 결찰후 집토끼 담낭상피세포의 미세구조적 변화" 한국전자현미경학회지 = Korean journal of electron microscopy 22 (2): 15~29    
    8. 1992 "머리에 방사선 상해를 받은 흰쥐 흑색질의 미세구조" 한국전자현미경학회지 = Korean journal of electron microscopy 22 (2): 30~45    
    9. 1993 "X-선 조사를 받은 흰쥐 망막의 색소상피세포와 기저복합층의 미세구조" 한국전자현미경학회지 = Korean journal of electron microscopy 23 (2): 11~26    
    10. 1993 "흰쥐 눈물샘의 Prolactin 존재에 관한 면역전자현미경적 연구" 한국전자현미경학회지 = Korean journal of electron microscopy 23 (1): 25~34    
  • 조휘동 (1)

  • 양남길 (15)

  • 안의태 (26)

  • 고정식 (35)

  • 김진국 (11)

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