본문 바로가기
HOME> 논문 > 논문 검색상세

논문 상세정보

Properties of Single $K^{+}$ Channels of Skeletal Muscle Incorporated into Planar Lipid Bilayer

Park, Jin-Bong   (College of Veterinary Medicine, Seoul National UniversityUU0000691  ); Kim, Hee-Jeong   (College of Veterinary Medicine, Seoul National UniversityUU0000691  ); Cho, Myung-Haing   (College of Veterinary Medicine, Seoul National UniversityUU0000691  ); Lee, Hang   (College of Veterinary Medicine, Seoul National UniversityUU0000691  ); Park, Hong-Ki   (Department of Chest Surgery, Graduate School, Gyeongsang National UniversityUU0000114  ); Lee, Mun-Han   (College of Veterinary Medicine, Seoul National UniversityUU0000691  ); Ryu, Pan-Dong   (College of Veterinary Medicine, Seoul National UniversityUU0000691  );
  • 초록

    single $K^{+}$ channels of skeletal muscle from the rat and frog were into planar lipid bilayers and their properties were studied. Fusion was induced by an osmotic gradient. Of the four types of $K^{+}$ channels recorded, the two most frequently observed were a voltage and $Ca^{2+}-activated$ $K^{+}$ channel and a $K^{+}$ channel with a prominent conductance substate. The first $K^{+}$ channel was identified as the large $Ca^{2+}-activated$ $K^{+}$ (BK) channel because the open-state probability was increased with depolarization (e-fold change per $10.6{\pm}3.5$ mV, n=8) and internal $Ca^{2+}$ (half-activation at $16.7{\pm}3.8$ mV, n=8, pCa 4) and its conductance was large ( $247{\pm}4.9$ pS, n=24 in 0.1 M KCI). Lifetime distributions of open- and closed-states could be fitted with single exponentials of several milliseconds. The mean open- and closed-lifetimes were linearly dependent on the intracellular $[Ca^{2+}]$ and $1/[Ca^{2+}]$ , respectively. The second $K^{+}$ channel showed a conductance substate at $30{\sim}60%$ of the open state. Its current-voltage relation was linear in the range of $-80\;{\sim}\;+80\;mV$ . The slope conductance of the substate and open-state were 40 and 144 pS in 0.2 M KCl, respectively. The channel was highly selective for $K^{+}$ over Cl. The open-state probability was weakly voltage-dependent (e-fold change per 35 mV. The lifetime distributions of open- and closed-states were fitted with two exponentials and the major gating occurred slowly at several hundred milliseconds. Based on the above results, we think the second type of $K^{+}$ channel is the sarcoplasmic reticulum $K^{+}$ (SRK) channel. In addition, both types of channel were also incorporated into the lipids extracted from the skeletal muscle. The channel properties recorded in the bilayers termed from synthetic and extracted lipids were qualitatively similar. Our data indicate that BK and SRK channels are rich in the skeletal muscle and their properties and regulation could be effectively studied in planar lipid bilayer.


  • 주제어

    Skeletal muscle .   $Large\ .   Ca^{2+}-activated\ .   K^{+}channel$ .   $Sarcoplasmic\ .   reticulum\ .   K^{+}\ .   channel$ .   Planar lipid bilayer.  

 저자의 다른 논문

  • Kim, Hee-Jeong (2)

    1. 1994 "쥐자궁근의 운동성에 대한 $K^+$채널 개방제의 이완 작용" The Korean journal of pharmacology : official journal of the Society of Pharmacology, Republic of Korea 30 (2): 191~203    
    2. 2010 "개의 큰 사강을 갖는 창상에서 무세포성 진피기질의 적용 3례" Journal of veterinary clinics = 한국임상수의학회지 27 (3): 299~301    
  • 조명행 (63)

  • 이항 (37)

  • Park, Hong-Gi (3)

  • 이문한 (42)

  • Ryu, Pan-Dong (37)

 활용도 분석

  • 상세보기

    amChart 영역
  • 원문보기

    amChart 영역

원문보기

무료다운로드
  • NDSL :
유료다운로드
  • 원문이 없습니다.

유료 다운로드의 경우 해당 사이트의 정책에 따라 신규 회원가입, 로그인, 유료 구매 등이 필요할 수 있습니다. 해당 사이트에서 발생하는 귀하의 모든 정보활동은 NDSL의 서비스 정책과 무관합니다.

원문복사신청을 하시면, 일부 해외 인쇄학술지의 경우 외국학술지지원센터(FRIC)에서
무료 원문복사 서비스를 제공합니다.

NDSL에서는 해당 원문을 복사서비스하고 있습니다. 위의 원문복사신청 또는 장바구니 담기를 통하여 원문복사서비스 이용이 가능합니다.

이 논문과 함께 출판된 논문 + 더보기