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Effects of Insulin and IGFS on Growth and Functional Differentiation in Primary Cultured Rabbit Kidney Proximal Tubule Cells -Growth and membrane transport-

Han, Ho-Jae   (Department of Veterinary Physiology, College of Veterinary Medicine, Chonnam National UniversityUU0001112  ); Park, Kwon-Moo   (Department of Veterinary Physiology, College of Veterinary Medicine, Chonnam National UniversityUU0001112  );
  • 초록

    The purpose of this study was to compare effects of insulin and IGFs on growth, apical membrane enzyme activities and membrane transport systems of primary cultured rabbit kidney proximal tubule cells. Results were as follows: 1. Insulin and IGF-I produced significant growth stimulatory effects at $5{\times}10^{-10}M.\;IGF-II(5×10^{-10}\;M)$ did not stimulate significant cell growth. 2. Insulin stimulated the phosphorylation of a 97 KD protein. It was difficult to determine whether this band represents insulin and/or the IGF-I receptor. 3. The activities of apical membrane enzymes (alkaline phosphatase, leucine aminopeptidase, and ${\gamma}-glutamyl \;transpeptidase)$ were observed to be diminished after the cells were placed in the culture environment. 4. The uptake of ${\alpha}-MG,$ Pi and Na was significantly increased in cells incubated with insulin or IGF-I, IGF-II had no effect on the uptake of these substrates. 5. Na-pump activity, as assayed by Rb uptake, was significantly increased in cells treated with insulin or IGFs. In conclusion, insulin and IGF-I exert stimulatory effects on growth and membrane transporter(glucose, Na, Pi, and Na-pump) activities in primary cultured rabbit kidney proximal tubule cells. IGF-II had no effect on cell growth and membrane transporter(glucose, Na and Pi) activities.


  • 주제어

    Kidney .   Insulin .   IGF .   Transporter.  

 저자의 다른 논문

  • Park, Kwon-Moo (8)

    1. 1994 "군용견(軍用犬) German shepherd의 hemoglobin 및 albumin형(型)에 관(關)한 연구(硏究)" 大韓獸醫學會誌 = Korean journal of veterinary research 34 (3): 487~492    
    2. 1996 "Effects of Insulin and IGFs on Phosphate Uptake in Primary Cultured Rabbit Renal Proximal Tubule Cells" 대한생리학회지 = Korean journal of physiology 30 (1): 63~76    
    3. 1996 "Effects of High Glucose Levels on the Protein Kinase C Signal Transduction Pathway in Primary Cultured Renal Proximal Tubule Cells" 대한생리학회지 = Korean journal of physiology 30 (2): 257~267    
    4. 1996 "호르몬 한정배지를 이용한 세포 초대배양계의 확립" 大韓獸醫學會誌 = Korean journal of veterinary research 36 (3): 551~563    
    5. 1996 "초대배양된 토끼 신장 근위세뇨관세포의 성장과 기능분화에 대한 insulin과 IGF의 효과 - Na+ uptake에 대한 IGF-I의 효과 -" 大韓獸醫學會誌 = Korean journal of veterinary research 36 (4): 783~794    
    6. 1997 "신장 근위세뇨관세포에서 고포도당이 IGF-I 결합과 포도당운반계에 미치는 영향" 大韓獸醫學會誌 = Korean journal of veterinary research 37 (2): 301~310    
    7. 1997 "초대배양한 신장 근위세뇨관세포에서 estradiol-17β와 IGF-I 수용체 발현과의 상관관계" 大韓獸醫學會誌 = Korean journal of veterinary research 37 (2): 311~319    
    8. 2002 "A Role of Mitogen Activated Protein Kinases and Inflammatory Responses in Gender Differences in Kidney Ischemia Injury" The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology 6 (3): 155~160    

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