본문 바로가기
HOME> 논문 > 논문 검색상세

논문 상세정보

Elfvingia applanata 엑스의 항균력 및 Naringenin과의 병용효과
Antimicrobial Activity of Elfvingia applanata Extract Alone and in Combination with Naringenin

천준희    (충북대학교 약학대학   ); 이순옥    (충북대학교 약학대학   ); 임교환    (충북대학교 약학대학   ); 어성국    (충북대학교 약학대학   ); 이종길    (충북대학교 약학대학   ); 한성순    (충북대학교 약학대학  );
  • 초록

    담자균류의 하나인 잔나비걸상 자실체로부터 얻은 수성엑스의 항세균 시험과 항진균 시험을 액체배지 희석법으로 실시하고 naringenin과의 병용시험을 checkerboard assay 법으로 평가하였다. 항세균 시험결과 전반적으로 그람음성균보다 그람양성균에 대해 비교적 양호한 항균력을 나타내었으나 사용된 균주중 그람음성균인 Proteus vulgaris 78615는 MIC가 1.125 mg/ml로 가장 양호한 항균효과를 나타내었다. 11종의 균주에 대해 실시된 항진균 시험결과 Candida albicans, Fusarium spp. 그리고 Trichophyton mentagraphytes의 MIC가 7.5 mg/ml로 다른 균주에 대한 항진균 효과보다 두배 더 강한 효과를 나타내었다. naringenin과의 병용시험에서 Staphylococcus aureus ATCC 25923에 대하여 부분적 상승효과를 나타내었고, Klebsiella pneumoniae ATCC 10031과 Salmonella typhi ATCC 6229 두 균주에서 상가효과가 관찰되었다. 그리고 Bacillus subtilis ATCC 6633을 비롯한 7종의 균주에서 무관효과를 나타내었으며 길항효과는 관찰되지 않았다.


    As part of our search for less toxic antimicrobial agents from natural resources, the antimicrobial activity of Elfvingia applanata $(P_{ers.})\;K_{arst.}$ extract was examined alone and in combination with naringenin. EA, the aqueous extract from the carpophores of E. applanata, was lyophilized and a dark brownish powder was obtained. Antimicrobial activity of EA was tested in vitro against nineteen strains of bacteria and eleven strains of fungi by serial broth dilution method, and expressed by minimal inhibitory concentration (MIC). Among nineteen strains of bacteria tested, the antimicrobial activity of EA was the most potent against Proteus vulgaris showing MIC of 1.125 mg/ml. EA also inhibited the growth of the selected fungi at higher concentrations ranging from 7.5 mg/ml to 15.0 mg/ml. To investigate the effect of antimicrobial combinations of EA with naringenin, the fractional inhibitory concentration index (FICI) was determined by checkerboard assay for each strain. The antimicrobial combinations of EA with naringenin resulted in partial synergism against Staphylococcus aureus only, and showed additive effect in two strains including Klebsiella pneumoniae and Salmonella typhi. Antagonism was not found.


  • 주제어

    Elfvingia applanata .   Carpophores .   Antimicrobial activity .   Minimal inhibitory concentration (MIC) .   Checkerboard assay .   Fractional inhibitory concentration index (FICI) .   Naringenin .   Antimicrobial combinations.  

  • 참고문헌 (34)

    1. Antibiotics from Basidiomycetes. 26, Phlebiakauranol aldehydean antifungal and cytotoxic metabolite from Punctularia alropurpurascens , Anke, H.;Casser, I.;Steglich, W. , J. Antibiotics / v.40,pp.443-449,
    2. Antibiotics from basidiomycetes. X. Scorodonin, a new antibacterial and antifungal metabolite from Marrassmius scorodonius (FR.) , Anke, T.;Kupa, J.;Schramm, G.;Steglich, W. , FR. J. antibiotics / v.33,pp.463-467,
    3. Prosthetic valve endocarditis by Staphylococcus epidermidis. Development of rifampin resistance during vancomycin and rifampin therapy , Chamovitz, B.;Bryant, R.E.;Gibert, D.N.;Harstein, A.I. , J.A.M.A. / v.253,pp.2867-2868,
    4. Studies on the constituents of the Higher Fungi of Korea (IV). An antibiotic component and a sterol of Coriolus sanguineus Fr. , Chung, K.S.;Shim, M.J.;Kim, B.K. , Arch. Pharm. Res. / v.10,pp.33,
    5. Antibacterial sesquiterpene arylester from Armillaria mellea , Donelly, D.;Abe, F.;Conveney, D.;Fukuda, N.;O'Reilly, J. , J. Natural Product. / v.48,pp.10-16,
    6. Antagonists of nucleic acid derivatives VIII. synergism in combination of biochemicaloly related metabolites , Elion, G.B.;Singer, S.;Hitchings, G.H. , J. Biol. Chem. / v.,pp.477-488,
    7. Acta Pathol. et Microbiol , Ericsson, H.;Sherris, J.C. , / v.,pp.,
    8. Mycology for the clinical Laboratory , Gary, S.M.;Douglas, M.J. , / v.,pp.262-266,
    9. Synergism between aminoglycosides and cephalosporins with antipseudomonal activity: interaction index and killing curve method , Hallander, H.O.;Dornbusch, L.;Gezelius, L.;Jacobson, K.;Karlsson, I. , Agents Chemother / v.22,pp.743-752,
    10. Studies on inorganic constituents and antimicrobial activity of Elfvingia applanata (Per) $K_{ARST}$ in Korea , Han, S.S.;Baik, S.R. , Chungbuk J. Pharm. Sci. / v.3,pp.44-50,
    11. Invitro interactions between fifampin and ampicillin or chloramphenicol against haemophilus influenzae , Jadavji, T.;Prober, C.G.;Cheung, R. , Agents Chemother / v.26,pp.91-93,
    12. The combined action of penicillin with streptomycin or chloromycetin on Enterococci in vitro , Jawetz, E.;Gunnison, J.B.;Colman, V.R. , Science / v.111,pp.254,
    13. Fungal nucleic acids as interferon inducers , Kandefer, S.M.;Kawecki, Z.;Guz, M. , Acta. Microbiol. Pol. / v.28,pp.277-291,
    14. A pictorial book of the Korean mushroom , Kim, S.S.;Kim, Y.B. , / v.,pp.298-299,
    15. Moxa compositions containing germanium , Kondo, A.;Kondo, K. , / v.,pp.,
    16. Antimicrobial combinations;Antibiotics in Laboratory Medicine , Krogsted, D.J.;Moellering, R.C.;Lorian, V.(ed.) , / v.,pp.537-595,
    17. Illustrated flora and fauna of Korea, 28(Mushroom) , Lee, J.Y.;Hong, S.W. , / v.,pp.366-367,
    18. Studies on the antibacterial activity of Poria cocos , Lee, K.S.;Lee, M.W.;Lee, J.Y. , Kor. J. Mycol. / v.10,pp.27-31,
    19. Therapy for experimental intraabdominal sepsis: Comparison of four cephalosprins with clindamycin plus gentamycin , Louie, T.J.;Onderdonk, A.B.;Gorbach, S.L.;Bartlett, J.G. , J. Infect. Dis. / v.135,pp.,
    20. Manufactures of a new ${\beta}-glucan$ , Misaki, A.;Sone, Y.;Yoshida, M.;Takeuchi, K. , / v.,pp.,
    21. Studies on the host-mediated antitumor polysaccharides. VI. Isolation and characterization of antitumor active ${\beta}-D-glucan $ from mycelial cells fo Ganoderma applanatum , Mizuno, T.;Ushiyama, M.;Usui, T.;Arakawa, M.;Shinkai, K.;Shimizu, M. , Shizuoka Daigaku Nogakubu Kenkyu Hokuku / v.32,pp.41-58,
    22. Effect of polysaccharides from Ganoderma applanatum on immune responses. I. Enhancing effect on the induction of delayed hypersensitivity in mice , Nakashima, S.;Umeda, Y.;Kanada, T. , Microbiol. Immunol. / v.23,pp.501-513,
    23. Production of milk-clotting enzymes by basidiomycetes , Nerud, F.;Misurcova, Z.;Musilek, V. , Folia Microbiol.(Proque) / v.34,pp.310-315,
    24. Bitter triterpenoids from the fungus Ganoderma applanatum , Nishitoba, T.;Goto, S.;Sato, H.;Sakamura, S. , Phytochemistry / v.28,pp.193-197,
    25. Antibacterial synergism. A proposal for chmotherapeutic potentiation between trimethoprim and sulfamethoxazole , Poe, M. , Science / v.194,pp.533,
    26. Triterpenes. LXIII. Triterpenes and steroids from Ganoderma applanatum , Protiva, J.;Skorkovska, H.;Urban, J.;Vystrcil, A. , Collect. Czech. Chem. Commun. / v.45,pp.2710-2713,
    27. Steroids from Ganoderma applanatum , Ripperger, H.;Budzikiewicz, H. , Phytochemistry / v.14,pp.2297-2298,
    28. Antibiotic substances from basidiomycetes. I , Robbins, W.J.;Kavanagh, F.;Hervey, A. , Pleurotus Proceeding of the National Academy of Science / v.33,pp.171-176,
    29. Efficacy and safty of aztreonam-clindamycin versus tobramycin-clindamycin in the treatment of lower respiratory tract infections caused by aerobic Gram-negative bacilli , Rodriguez, J.R.;Ramirez Ronda, C.H.;Nevarez, M. , Antimicrob. Agents Chemother / v.27,pp.246-251,
    30. The emergence of isoiazidresistant cultures in patients with pulmonary tuverculosis during treatment with isoniazid alone or isoniazid plus PAS , Selkon, J.B.;Devadatta, S.;Kulkarnia, K.G.;Mitchison, D.A.;Narayana, A.S.L.;Narayana Nair, C.;Ramachandran, K. , Bull. W.H.O. / v.31,pp.273-294,
    31. Isolation and identification of ergosterol peroxide from Cetraria richardsonii and Ganoderma applanatum , Svridonov, V.N.;Strigina, L.I. , Khim. Prir. Soedin. / v.5,pp.669,
    32. Antibiotics in Laboratory Medicine , Lorian, V.M.D. , / v.,pp.537-545,
    33. Chemotherapy of tuberculosis;Pharmacological Basis of Therapeutics, 5 , Weistein, L.;Goodman, L.S.(ed.);Gilman, A.(ed.) , / v.,pp.1214,
    34. Chemical constituents of fungi. II. Coenzymes $Q_9$ from Ganoderma applanatum , Yamada, Y.;Hsu, C.S.;Iguchi, K.;Suzuki, M. , Tohoku Yakka Daigaku Kenkyu Nempo / v.23,pp.290-292,

 저자의 다른 논문

  • 임교환 (4)

    1. 1994 "Elfvingia applanata 엑스가 수종 항생물질의 항균력에 미치는 병용효과" 약학회지 = Yakhak hoeji 38 (6): 742~748    
    2. 1996 "천연 Rutin의 항균효과와 급성독성에 미치는 영향" 생약학회지 27 (4): 309~315    
    3. 1999 "잔나비걸상 수용성물질의 Vesicular Stomatitis Virus(New Jersey Serotype)에 대한 항바이러스작용과 Interferon과의 병용효과" 한국균학회지 = The Korean journal of mycology 27 (2): 175~179    
    4. 1999 "잔나비걸상버섯 수용성물질의 항바이러스효과" 생약학회지 30 (1): 25~33    
  • Lee, Chong-Kil (63)

  • Han, Seong-Sun (33)

 활용도 분석

  • 상세보기

    amChart 영역
  • 원문보기

    amChart 영역

원문보기

무료다운로드
  • NDSL :
유료다운로드

유료 다운로드의 경우 해당 사이트의 정책에 따라 신규 회원가입, 로그인, 유료 구매 등이 필요할 수 있습니다. 해당 사이트에서 발생하는 귀하의 모든 정보활동은 NDSL의 서비스 정책과 무관합니다.

원문복사신청을 하시면, 일부 해외 인쇄학술지의 경우 외국학술지지원센터(FRIC)에서
무료 원문복사 서비스를 제공합니다.

NDSL에서는 해당 원문을 복사서비스하고 있습니다. 위의 원문복사신청 또는 장바구니 담기를 통하여 원문복사서비스 이용이 가능합니다.

이 논문과 함께 출판된 논문 + 더보기